Abreviews for loading control antibodies

Abreviews - our unique customer review system. Find out exactly how our antibodies have worked for other scientists before you buy!

Read our Abreviews for the different types of loading control antibody:

Beta Actin loading control antibodies

VDAC1 Mitochondrial loading control antibodies

Tubulin loading control antibodies

COXIV Mitochondrial loading control antibodies

GAPDH loading control antibodies

TBP Nuclear loading control antibody

For each Abreview you submit you can earn between 50 and 300 Abpoints. The Abpoints you earn is based on submission time and type of application. The less characterised the product you review, the more Abpoints you will receive. If you submit an image with your Abreview you will earn an extra 100 Abpoints!

More about Abpoints

More about Abreviews


Abreviews for beta Actin Cytoplasmic / Whole Cell Loading Control antibodies

Review Icon Anonymous Abreview submitted on 31 October 2006

WB - beta Actin antibody (ab8226)

WB - beta Actin antibody (ab8226)

Cell lysate from isolated rat striatum.
Lane 1. Left striatum
Lane 2. Right striatum
Lane 3. Cell lysate from HEK 293 cells

Sample

Human Cell lysate - whole cell (Prostate Cancer cell line)

Loading amount:

10000 cell numbers/lane

Application

Western Blot

Conditions:

Reduced

Dilution

1/500

Rating

Fantastic! - I would use it again and recommend it 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

Review Icon Anonymous Abreview submitted on 11 May 2006

Sample

Dog Cell lysate - whole cell (MDCK cells)

Loading amount:

30 µg/lane

Application

Western Blot

Conditions:

Reduced

Dilution

1/2500

Rating

Fantastic! - I would use it again and recommend it 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

Additional notes

RIPA lysis buffer with Protease Inhibitor Cocktail (Sigma) was used. The protein was transfered to the nitrocellulose membranes.
Peroxidase-Labeled Affinity Purified Secondary Antibody to Rabbit IgG (KPL) was used and visualization of beta actin bands on the membranes will be done by using 1 Component TMB Membrane Proxidase Substrate (KPL).

Read all Abreviews for beta Actin antibody (ab8226) (20 Abreviews)

WB - beta Actin antibody (ab8226)

WB - beta ACTIN2

Review Icon  Anonymous Abreview submitted on 19 June 2006

Sample

Mouse Tissue lysate - whole (Brain and Spinal Cord)

Loading amount:

2 µg/lane

Application

Western Blot

Conditions:

Non-reduced

Dilution

1/1000

Rating

Fantastic! - I would use it again and recommend it 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

Read all Abreviews for beta Actin antibody (ab8227) (29 Abreviews)

Review Icon  Anonymous Abreview submitted on 25 April 2006

Sample

Human Tissue lysate - whole (Brain, Heart, Small Intestine, Kidney, Liver, Lung)

Loading amount:

20 µg/lane

Application

Western Blot

Conditions:

Reduced

Dilution

1/1000

Rating

Good - I would definitely use it again 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

Read all Abreviews for beta Actin antibody [mAbcam 8224] (ab8224) (2 Abreviews)

All beta Actin Loading Control antibodies


WB - alpha Tubulin antibody (ab7291)

WB - ab7291
Abreviews for Tubulin Cytoplasmic / Whole Cell Loading Control antibodies

Review Icon  Abreview by Kirk McManus submitted on 24 February 2006

Sample

Human Cell lysate - whole cell (HeLa)

Application

Western Blot

Conditions:

Reduced

Dilution

1/10000

Rating

Fantastic! - I would use it again and recommend it 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

Additional notes

This antibody works extremely well at 1:10,000 and could probably be diluted to 1:20,000 and still provide excellent signal. 

Read all Abreviews for alpha Tubulin antibody [DM1A] (ab7291)
(5 Abreviews)

All Tubulin Loading Control antibodies


Abreviews for GAPDH Cytoplasmic / Whole Cell Loading Control antibodies

Review Icon Anonymous Abreview submitted on 8 June 2006

Sample

Human Cell lysate - other (human ovarian cancer cell lines)

Loading amount:

20 µg/lane

Application

Western Blot

Conditions:

Reduced

Dilution

1/2000

Rating

Fantastic! - I would use it again and recommend it 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

Read all Abreviews for GAPDH antibody (ab9485) (24 Abreviews)

Review Icon Anonymous Abreview submitted on 14 October 2005

Sample

Human Cell lysate - whole cell (T cells)

Application

Western Blot

Conditions:

Reduced

Dilution

1/2000

Rating

Fantastic! - I would use it again and recommend it 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

Additional notes

I used a 4-12% Bis-Tris gel, hybond ECL nitrocellulose mebrane and MOPS buffer. In the left lane is T cell lysate, the right lane contains Jurkat lysate

Read all Abreviews for GAPDH antibody [mAbcam 9484] (HRP) (ab9482) (7 Abreviews)

All GAPDH Loading Control antibodies

WB - GAPDH antibody (ab9485)

WB - ab9485


Abreviews for COXIV Mitochondrial Loading Control antibodies

Review Icon Anonymous Abreview submitted on 15 September 2006

Sample

Mouse tissue lysate (pancreatic islets)

Application

Western Blot

Conditions:

Reduced

Dilution

1/1000

Rating

Good - I would definitely use it again 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

Additional notes

Incubation was done at 4 degree C. 

No Abreviews have yet been submitted for these Loading Controls...

... Be the first to tell us how they work and earn 300 Abpoints!

View all COXIV Mitochondrial Loading Control antibodies

Learn more about Abreviews and Abpoints


Abreviews for VDAC1 Mitochondrial Loading Control antibodies

Review Icon Anonymous Abreview submitted on 15 September 2006

Sample

Mouse tissue lysate (pancreatic islets)

Application

Western Blot

Conditions:

Reduced

Dilution

1/1000

Rating

Good - I would definitely use it again 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

Additional notes

Incubation was done at 4 degree C. 

Read all Abreviews for VDAC1 / Porin antibody (ab3434) (3 Abreviews)

No Abreviews have yet been submitted for these Loading Controls

... Be the first to tell us how they work and earn 300 Abpoints!

View all VDAC1 Mitochondrial Loading Control antibodies

Learn more about Abreviews and Abpoints


Abreviews for TBP Nuclear Loading Control antibody

Review Icon review by Jilin Liu submitted on 19 August 2004 (old style)

Application

Western blot

Notes

Western Blot
To detect endogenous TBP in Rat ROS 17/2.8 cells, a rat osteosarcoma cell line.
Worked very well

Dilution notes

1:2000