Choose ELISPOT kits for the analysis of cytokines and other soluble molecule production and secretion from T-cells at a single cell level.
FLUOROSPOT kits offer the same application, but with the added flexibility of fluorescence detection.
Using sandwich ELISA technology, ELISPOT and Dual ELISPOT can detect secreted cytokines and measure the frequency of single cells that simultaneously produce multiple cytokines or other effector molecules. Single and Dual FLUOROSPOT kits are also available to give you flexibility in studying the relationship between two proteins.
Now you can detect individual cells secreting the target protein of interest in response to various drugs, stimuli or inhibitors. Our kits will span your research into Th1/Th2 response, vaccine development, viral infection monitoring, infectious diseases, autoimmune diseases and transplantation, in addition to a growing number of other kits in our catalogue.
ELISPOT and FLUOROSPOT assays provide:
- High sensitivity
- Fast procedure
- Accuracy and reliability
- No cross reactivity
- Dual detection allowing investigation into the interaction of two proteins
ELISPOT and FLUOROSPOT assays allow visualization of the secretory product of individual activated or responding cells. Each spot that develops in the assay represents a single reactive cell. Thus, the ELISPOT and FLUOROSPOT assays provide both qualitative (type of immune protein) and quantitative (number of responding cells) information.
» view ELISPOT protocol overview.
» view ELISPOT and FLUOROSPOT brochure.
» view ELISPOT and Dual ELISPOT protocols together with ELISPOT troubleshooting tips.
ELISPOT and FLUOROSPOT kits are available in a number of formats from pre-coated kits for easy analysis and increased throughput to Antibody Matched Pairs.
