DyLight® Fluorochrome conjugated secondary antibodies

An alternative to conventional dyes

Our catalog has been broadened to include secondary antibodies conjugated with DyLight® 488, DyLight® 550, DyLight® 594 and DyLight® 650.

Learn more about the characteristics and advantages of using DyLight® fluorochromes:

View some of our secondary antibodies conjugated with:

 info icon Download (3MB) DyLight® conjugated secondary antibodies a PDF version of this page or email us to receive your free hard copy today.

 

New family of dyes

DyLight® fluorochromes exhibit higher fluorescence intensity, photostability and water solubility and remain fluorescent from pH 4 to pH 9. Additionally, the water solubility of the DyLight® fluorochromes allows a high dye-to-antibody ratio to be achieved without causing precipitation of conjugates.

DyLight® fluorochromes have absorption maxima ranging from 350nm to 777nm, covering the entire visible light spectrum. Both the absorption and emission properties of the DyLight® fluorochomes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation.

 

Expand the emission spectra of Dylight® fluorochromes

Figure: Emission spectra of DyLight® fluorochromes available in our catalog

DyLight® 488

DyLight® 550

DyLight® 594

DyLight® 650

In order to expand the spectrum, please click on the DyLight® image.


Advantages

  • Bright fluorescence – intense emission provides superior sensitivity
  • Narrow emission spectra – negligible bleed-through between fluorochrome channels makes multi-color imaging easy
  • Highly photostable – better resistance to photobleaching
  • Completely stable in buffers ranging from pH 4-9
  • Instrument compatibility – DyLight® can be visualized with common laser and filter settings

 

DyLight® dye properties

DyLight® fluorochromes are suitable for fluorescence microscopy, flow cytometry and Western blotting (with fluorescent scanners).

 

Table: Spectral properties of DyLight® fluorochromes. In order to expand the spectra please click on the spectrum icons.

Fluorochrome

Color

Ex/Em

Spectra

ε (M-1cm-1)

Spectrally equivalent dyes

DyLight® 488

green

493/518

Expand the Dylight®488 spectrum

70K

Fluorescein, FITC, Cy2®

DyLight® 550

 Yellow

562/576

Expand the Dylight®550 spectrum

150K

Cy3®, TRITC

DyLight® 594

 orange

593/618

Expand the Dylight®594 spectrum

80K

Texas Red®

DyLight® 650

 red

652/672

Expand the Dylight®650 spectrum

250K

Cy5®

Ex/Em: Excitation and emission wavelength in nanometers (+/- 4nm)

ε: Molar extinction coefficient at the absorption maximum

For further information or help, contact our Scientific Support team.

 

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Cy® and CyDye® are trademarks of GE Healthcare Limited.


Back to secondary antibody resources