Our catalog has been broadened to include secondary antibodies conjugated with DyLight® 488, DyLight® 550, DyLight® 594 and DyLight® 650.
Learn more about the characteristics and advantages of using DyLight® fluorochromes:
View some of our secondary antibodies conjugated with:
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New family of dyes
DyLight® fluorochromes exhibit higher fluorescence intensity, photostability and water solubility and remain fluorescent from pH 4 to pH 9. Additionally, the water solubility of the DyLight® fluorochromes allows a high dye-to-antibody ratio to be achieved without causing precipitation of conjugates.
DyLight® fluorochromes have absorption maxima ranging from 350nm to 777nm, covering the entire visible light spectrum. Both the absorption and emission properties of the DyLight® fluorochomes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation.
Figure: Emission spectra of DyLight® fluorochromes available in our catalog
— DyLight® 488
— DyLight® 550
— DyLight® 594
— DyLight® 650
In order to expand the spectrum, please click on the DyLight® image.
Advantages
- Bright fluorescence – intense emission provides superior sensitivity
- Narrow emission spectra – negligible bleed-through between fluorochrome channels makes multi-color imaging easy
- Highly photostable – better resistance to photobleaching
- Completely stable in buffers ranging from pH 4-9
- Instrument compatibility – DyLight® can be visualized with common laser and filter settings
DyLight® dye properties
DyLight® fluorochromes are suitable for fluorescence microscopy, flow cytometry and Western blotting (with fluorescent scanners).
Table: Spectral properties of DyLight® fluorochromes. In order to expand the spectra please click on the spectrum icons.
| Fluorochrome | Color | Ex/Em | Spectra | ε (M-1cm-1) | Spectrally equivalent dyes |
|---|---|---|---|---|---|
| DyLight® 488 |  | 493/518 |  | 70K | Fluorescein, FITC, Cy2® |
| DyLight® 550 |  | 562/576 |  | 150K | Cy3®, TRITC |
| DyLight® 594 |  | 593/618 |  | 80K | Texas Red® |
| DyLight® 650 |  | 652/672 |  | 250K | Cy5® |
Ex/Em: Excitation and emission wavelength in nanometers (+/- 4nm)
ε: Molar extinction coefficient at the absorption maximum
For further information or help, contact our Scientific Support team.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Cy® and CyDye® are trademarks of GE Healthcare Limited.
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