Abcam's range of pre-adsorbed secondary antibodies
- Pre-adsorbed Alexa Fluor® 488/555/594/647 conjugated secondary antibodies
- Pre-adsorbed Cy3® conjugated secondary antibodies
- Pre-adsorbed Cy5® conjugated secondary antibodies
- Pre-adsorbed DyLight® conjugated secondary antibodies
Pre-adsorbed secondary antibodies are ideal for multi-color imaging and IHC experiments when several primary antibodies and their corresponding secondary antibodies are used simultaneously. Reducing the risk of cross-reactivity between the secondary antibody and endogenous immunoglobulins present on cell and tissue samples. The secondary antibody should therefore, be pre-adsorbed against the same species the sample originated from. For example, it is advisable to use a secondary antibody pre-adsorbed against human serum when staining human tissues or cell lines.
Figure1: Pre-adsorption procedure:
Figure 1: Pre-adsorption (also referred to as cross-adsorption) is an extra step to increase the specificity of an antibody. Antibodies (in this example the mixture of purple antibodies recognizing rabbit IgG light-chains) are passed through a matrix containing immobilized serum proteins from potentially cross-reactive species (in this example antibodies that recognize sheep and bovine light-chains). Only antibodies specific to rabbit IgG-light chains will pass through the column. Antibodies cross-reacting with sheep or bovine IgG light-chains will bind and stay adsorbed to the matrix. The result of the procedure is a more specific antibody, which is especially useful in IHC when cross-reactivity is feared.
Cy3® conjugated secondary antibodies (pre-adsorbed against up to eight species)
Product name | Applications | Antibody code |
|---|---|---|
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC-P | ||
IHC,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF |
Cy5® conjugated secondary antibodies (pre-adsorbed against up to eight species)
Product name | Applications | Antibody code |
|---|---|---|
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF,WB | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF, ELISA | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF | ||
IHC,Flow Cyt,ICC/IF | ||
IHC-P,Flow Cyt,ICC/IF |
DyLight® conjugated secondary antibodies - pre-adsorbed against different species
Pre-adsorption against Human and Mouse
| Product name | Applications | Antibody code |
|---|---|---|
| IHC-P,Flow Cyt,ICC/IF | ||
| IHC-P,Flow Cyt,ICC/IF | ||
| IHC-P,Flow Cyt,ICC/IF |
Pre-adsorption against Human, Rat and Mouse
| Product name | Applications | Antibody code |
|---|---|---|
| IHC-P,Flow Cyt,ICC/IF | ||
| IHC-P,Flow Cyt,ICC/IF | ||
| IHC-P,Flow Cyt,ICC/IF | ||
| IHC-P,Flow Cyt,ICC/IF |
Pre-adsorption against Chicken, Cow, Goat, Horse, Human, Rabbit, Rat and Sheep
| Product name | Applications | Antibody code |
|---|---|---|
| IHC-P,Flow Cyt,ICC/IF | ||
| IHC-P,Flow Cyt,ICC/IF | ||
| IHC-P,Flow Cyt,ICC/IF | ||
| IHC-P,Flow Cyt,ICC/IF |
Learn more about the characteristics and advantages of using DyLight® fluorochromes.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.
Back to secondary antibody resources
