Pre-adsorption process

Secondary antibodies selected for their minimal cross-reactivity with serum proteins of non-related species.

Abcam's range of pre-adsorbed secondary antibodies

  1. Pre-adsorbed Alexa Fluor® 488/555/594/647 conjugated secondary antibodies
  2. Pre-adsorbed Cy3® conjugated secondary antibodies
  3. Pre-adsorbed Cy5® conjugated secondary antibodies
  4. Pre-adsorbed DyLight® conjugated secondary antibodies

Pre-adsorbed secondary antibodies are ideal for multi-color imaging and IHC experiments when several primary antibodies and their corresponding secondary antibodies are used simultaneously. Reducing the risk of cross-reactivity between the secondary antibody and endogenous immunoglobulins present on cell and tissue samples. The secondary antibody should therefore, be pre-adsorbed against the same species the sample originated from. For example, it is advisable to use a secondary antibody pre-adsorbed against human serum when staining human tissues or cell lines.

Figure1: Pre-adsorption procedure:

Pre-adsorption procedure

Figure 1: Pre-adsorption (also referred to as cross-adsorption) is an extra step to increase the specificity of an antibody. Antibodies (in this example the mixture of purple antibodies recognizing rabbit IgG light-chains) are passed through a matrix containing immobilized serum proteins from potentially cross-reactive species (in this example antibodies that recognize sheep and bovine light-chains). Only antibodies specific to rabbit IgG-light chains will pass through the column. Antibodies cross-reacting with sheep or bovine IgG light-chains will bind and stay adsorbed to the matrix. The result of the procedure is a more specific antibody, which is especially useful in IHC when cross-reactivity is feared.

Cy3® conjugated secondary antibodies (pre-adsorbed against up to eight species)

Product name

Applications

Antibody code

Goat polyclonal to Chicken IgY - H&L (Cy3®)

IHC-P,Flow Cyt,ICC/IF

ab97145

Donkey polyclonal to Goat IgG - H&L (Cy3®)

IHC,Flow Cyt,ICC/IF

ab6949

Donkey polyclonal to Goat IgG - H&L(Cy3®)

IHC-P,Flow Cyt,ICC/IF

ab97115

Goat polyclonal to Guinea Pig IgG - H&L (Cy3®)

IHC-P,Flow Cyt,ICC/IF

ab102370

Goat polyclonal to Human IgG - H&L (Cy3®)

IHC-P,Flow Cyt

ab6957

Goat polyclonal to Human IgG - H&L (Cy3®)

IHC-P,Flow Cyt,ICC/IF

ab97170

Goat polyclonal to Mouse IgG - H&L (Cy3®)

IHC-P,Flow Cyt,ICC/IF

ab97035

Sheep F(ab')2 polyclonal to Mouse IgG - H&L (Cy3®)

IHC-P

ab50502

Goat polyclonal to Rabbit IgG - H&L (Cy3®)

IHC,Flow Cyt,ICC/IF

ab6939

Goat polyclonal to Rabbit IgG - H&L (Cy3®)

IHC-P,Flow Cyt,ICC/IF

ab97075

Goat polyclonal to Rat IgG - H&L (Cy3®)

IHC,Flow Cyt,ICC/IF

ab6953

Goat polyclonal to Rat IgG - H&L (Cy3®)

IHC-P,Flow Cyt,ICC/IF

ab98416

Goat polyclonal to Syrian Hamster IgG - H&L (Cy3®)

IHC,Flow Cyt,ICC/IF

ab6969

Goat polyclonal to Syrian Hamster IgG - H&L (Cy3®)

IHC-P,Flow Cyt,ICC/IF

ab102318

 

Cy5® conjugated secondary antibodies (pre-adsorbed against up to eight species)

Product name

Applications

Antibody code

Goat polyclonal to Chicken IgY - H&L (Cy5®)

IHC-P,Flow Cyt,ICC/IF

ab97147

Donkey polyclonal to Goat IgG - H&L (Cy5®)

IHC,Flow Cyt,ICC/IF,WB

ab6566

Donkey polyclonal to Goat IgG - H&L (Cy5®)

IHC-P,Flow Cyt,ICC/IF

ab97117

Goat polyclonal to Guinea Pig IgG - H&L (Cy5®)

IHC,Flow Cyt,ICC/IF

ab6567

Goat polyclonal to Guinea Pig IgG - H&L (Cy5®)

IHC-P,Flow Cyt,ICC/IF

ab102372

Goat polyclonal to Human IgG - H&L (Cy5®)

IHC-P,Flow Cyt,ICC/IF

ab97172

Goat polyclonal to Mouse IgG - H&L (Cy5®)

IHC,Flow Cyt,ICC/IF, ELISA

ab6563

Goat polyclonal to Mouse IgG - H&L (Cy5®)

IHC-P,Flow Cyt,ICC/IF

ab97037

Goat polyclonal to Rabbit IgG - H&L (Cy5®)

IHC,Flow Cyt,ICC/IF

ab6564

Goat polyclonal to Rabbit IgG - H&L (Cy5®)

IHC-P,Flow Cyt,ICC/IF

ab97077

Goat polyclonal to Rat IgG - H&L (Cy5®)

IHC,Flow Cyt,ICC/IF

ab6565

Goat polyclonal to Rat IgG - H&L (Cy5®)

IHC-P,Flow Cyt,ICC/IF

ab98418

Goat polyclonal to Syrian Hamster IgG - H&L (Cy5®)

IHC,Flow Cyt,ICC/IF

ab6568

Goat polyclonal to Syrian Hamster IgG - H&L (Cy5®)

IHC-P,Flow Cyt,ICC/IF

ab102320

 

DyLight® conjugated secondary antibodies - pre-adsorbed against different species


Pre-adsorption against Human and Mouse

Product name

Applications

Antibody code

Goat polyclonal to Rat IgG - Fc specific (DyLight® 488)

IHC-P,Flow Cyt,ICC/IF

ab96971

Goat polyclonal to Rat IgG - Fc specific (DyLight® 594)

IHC-P,Flow Cyt,ICC/IF

ab96973

Goat polyclonal to Rat IgG - Fc specific (DyLight® 650)

IHC-P,Flow Cyt,ICC/IF

ab96974

 

Pre-adsorption against Human, Rat and Mouse

Product name

Applications

Antibody code

Goat polyclonal to Rabbit IgG - H&L (DyLight® 488)

IHC-P,Flow Cyt,ICC/IF

ab96895

Goat polyclonal to Rabbit IgG - H&L (DyLight® 550)

IHC-P,Flow Cyt,ICC/IF

ab96896

Goat polyclonal to Rabbit IgG - H&L (DyLight® 594)

IHC-P,Flow Cyt,ICC/IF

ab96897

Goat polyclonal to Rabbit IgG - H&L (DyLight® 650)

IHC-P,Flow Cyt,ICC/IF

ab96898

 

Pre-adsorption against Chicken, Cow, Goat, Horse, Human, Rabbit, Rat and Sheep

Product name

Applications

Antibody code

Goat polyclonal to Mouse IgG - H&L (DyLight® 488)

IHC-P,Flow Cyt,ICC/IF

ab96879

Goat polyclonal to Mouse IgG - H&L (DyLight® 550)

IHC-P,Flow Cyt,ICC/IF

ab96880

Goat polyclonal to Mouse IgG - H&L (DyLight® 594)

IHC-P,Flow Cyt,ICC/IF

ab96881

Goat polyclonal to Mouse IgG - H&L (DyLight® 650)

IHC-P,Flow Cyt,ICC/IF

ab96882

 

Learn more about the characteristics and advantages of using DyLight® fluorochromes.

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.


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