We have blocking buffers and reagents to support your own immunoassay development including blocking, stability and detection reagents.
Additionally, we offer TMB (3,3’,5,5’-Tetramethylbenzidine), the most popular chromogenic substrate for HRP detection with different kinetic formulations to help optimize your particular application.
Blocking buffers and conjugate stabilizers
The buffers are specifically designed to preserve the conformational spatial arrangement of proteins in their native folded state when dried or in solution ensuring the biological activity of the protein.
Product name | Function | Product code |
---|---|---|
Immunoassay Blocking Buffer | Blocking agent that can be dried down or used for same-day assays. Preserves the conformation and activity of dried antibodies, proteins or enzymes coated to a surface. | ab171534 |
Immunoassay Blocking Buffer (BSA free) | Blocking solution without BSA. Synthetic formulation to eliminate background issues. | ab171535 |
AP Stability Conjugate Reagent | Preserves AP conjugated proteins in native folded state in solution | ab171536 |
HRP Stability Conjugate Reagent | Preserves HRP conjugated proteins in native folded state in solution | ab171537 |
Table 1: Buffers to preserve the conformational spatial arrangement of proteins
See below some comparative studies when using our buffers with monoclonal or polyclonal antibodies.
Figure 1: After 30 months in Immunoassay Blocking Buffer (ab171534) at room temperature, the monoclonal antibody retained 70% activity and the polyclonal antibody retained 95% activity.
Figure 2: After 30 months in Immunoassay Blocking and Protein Stabilizer (BSA free) (ab171535) at room temperature, the monoclonal antibody retained greater than 85% activity and the polyclonal antibody retained 90% activity.
Product name | Product code | Stabilization | ELISA | WB | Microspheres |
---|---|---|---|---|---|
Immunoassay Blocking Buffer | ü | ü | ü | ||
Immunoassay Blocking and Protein Stabilizer (BSA free) | ü | ü | ü | ü | |
AP Stability Conjugate Reagent | ü | ||||
RP Stability Conjugate Reagent | ü |
Table 2: Blocking and conjugate buffers guide
Different kinetic TMB formulations
The choice of the TMB substrate formulation optimizes the ELISA dynamic range as well as the assay sensitivity:
- Detection of low concentration proteins of interest over a short period of time
- Improved assay sensitivity and performance
- Reliable standard curve
Product name | Function | Product code |
---|---|---|
TMB ELISA substrate (Highest Sensitive) | Most sensitive TMB formulation | ab171522 |
TMB ELISA substrate (High Sensitivity) | Formulated for liquid level detection systems on instruments | ab171523 |
TMB ELISA substrate (Fast Kinetic Rate) | Most popular and robust formulation | ab171524 |
TMB ELISA substrate (Slow Kinetic Rate) | Allow a wide dynamic/working range without compromising the assay sensitivity | ab171525 |
TMB ELISA substrate (Slower Kinetic Rate) | Option for maximum dynamic/working range | ab171526 |
TMB ELISA substrate (Slowest Kinetic Rate) | Option for extended dynamic range and improved reproducibility | ab171527 |
Table 3: Range of TMB substrates with different kinetic formulations
Figure 3: View the kinetics among different TMB formulations after a sandwich ELISA where the capture antibody was blocked with Immunoassay Blocking Buffer (ab171534) and the HRP conjugated detection antibody was diluted with HRP Stability Conjugate Reagent (ab171537).