MitoSciences® | In-Cell ELISA

A leading supplier of mitochondrial research tools

Advancing mitochondrial research and developing products that support critical research areas in cancer, neurodegeneration and metabolic disorders.

  1. In-Cell ELISA kits
  2. Antibodies tested in In-Cell ELISA

1. In-Cell ELISA kits

In-Cell ELISA (ICE) allows quantitative comparisons of many proteins simultaneously in whole cells under different conditions and treatments.

Figure 3. Expand the image to view In-Cell ELISA technology and benefits

The In-Cell ELISA principle is similar when using either IR or colormetric detection methods. After cells have been grown to approximately 80% confluency in a 96- or 384-well plate, a drug or other treatment is applied to stimulate a cellular response. After treatment the cells are fixed and permeabilized in the wells, effectively "freezing" the cells in context with no further sample preparation perturbations. Primary antibodies are then added which bind to their intended targets within the mitochondria or other subcellular compartment. After incubation, the unbound primary antibodies are washed away and secondary antibodies are added. These secondaries are conjugated to either IRDyes® or to an enzyme label (HRP or AP) for the colorimetric versions of the assays. Unbound secondaries are washed away, reaction buffer is added for the colorimetric assays and the signal is read on a suitable instrument for the kit type.

Support packs are also available including all these premade buffers, for performing ICE with adherent and non adherent cells. The support pack contains a specialized cell culture plate ideal for experiments with adherenet and non-adherent cells or cells which may detach due to treatment.

NameReacts withAmountsMitoSciences codeAbcam code

AKT total + Phospho s473 In-Cell ELISA Kit (Colormetric)

Hu

1 x 96 tests

MS1047

ab126578

AKT total + Phospho s473 In-Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1004

ab126579

AKT total + Phospho s473 In-Cell ELISA Kit (Fluorescent)

Hu

2 x 96 tests

-

ab156029

AMPKa Total and Phospho T172 ICE Kit (IR)

Hu, Ms, Rt

1 x 96 tests

-

ab151280

Cell Cycle Human In-Cell ELISA Kit (IR)

Hu, Ms

1 x 96 tests

MS1124

ab139412

Cell Cycle Human In-Cell ELISA Kit (Fluorescent)

Hu, Ms

1 x 96 tests

-

ab140363

Fatty Acid Oxidation Human In-Cell ELISA Kit

Hu

1 x 96 tests

MS975

ab118182

GSK3 beta Total + pSer9 Human In-Cell ELISA Kit (Colorimetric)

Hu

1 x 96 tests

MS1081

ab129731

GSK3 beta Total + pSer9 Human In-Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1006

ab129730

Hif1 alpha + BNIP3 Hypoxia Human In Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1060

ab129733

Hif1 + GLUT Hypoxia Human In Cell ELISA Kit

Hu

1 x 96 tests

MS1050

ab125298

Hif1 + PDK1 Hypoxia Human In Cell ELISA Kit

Hu

1 x 96 tests

MS1008

ab125299

Hif1 + Actin Hypoxia Human In Cell ELISA Kit

Hu

1 x 96 tests

MS1049

ab125300

H2A.X pSer139 + Cleaved-PARP In Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1098

ab131383

H2A.X Total + pSer139 In Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1099

ab131382

ICE (In-Cell ELISA) Support Pack

5 x 96 tests

MS922

ab111542

ICE (In-Cell ELISA) Support Pack w/o plates

5 x 96 tests

MS921

ab111541

MitoBiogenesis™ In-Cell ELISA Kit (IR)

Cow, Hu, Ms, Rat

2 x 96 tests

MS642

ab110216

MitoBiogenesis™ In-Cell ELISA Kit (Colorimetric)

Cow, Hu, Ms, Rat

2 x 96 tests

MS643

ab110217

MitoBiogenesis™ In-Cell ELISA Kit (Fluorescent)

Cow, Hu, Ms, Rat

1 x 96 tests

-

ab140359

mTOR (Total + pSer2448) In-Cell ELISA Kit (IR)

Hu

1 x 96 tests

-

ab168539

p53 Total + pSer46 Human In-Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1018

ab128570

p53 Total + pSer392 Human In-Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1063

ab128571

p53 Total + pSer9 Human In-Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1108

ab135393

RSK1 p90 & pSer380 In-Cell ELISA (Fluorescent)

Hu, Ms

1 x 96 tests

-

ab170196

PARP-1 (cleaved) In-Cell ELISA Kit (IR)

Hu

2 x 96 tests

MSA43

ab110215

PARP-1 (cleaved) In-Cell ELISA Kit (Colorimetric)

Hu

1 x 96 tests

MS1121

ab139410

PARP-1 (cleaved) In-Cell ELISA Kit (Fluorescent)

Hu

1 x 96 tests

-

ab140362

PhosphoPDH In-Cell ELISA Kit (Colorimetric)

Cow, Hu, Ms, Rat

2 x 96 tests

MSP48

ab110219

PhosphoPDH In-Cell ELISA Kit (IR)

Cow, Hu, Ms, Rat

2 x 96 tests

MSP47

ab110218

RSK1 p90 Total & pSer380 In-Cell ELISA Kit (IR)

Hu, Ms

1 x 96 tests

-

ab157398

SIRT1 Total + pSer47 Human In-Cell ELISA Kit (IR)

Hu

1 x 96 tests

MS1110

ab135394

SIRT1 Total + pSer47 Human In-Cell ELISA Kit (Colorimetric)

Hu

1 x 96 tests

MS1114

ab136807

SIRT1 Total + pSer47 Human In-Cell ELISA Kit (Fluorescent)

Hu

1 x 96 tests

MS1115

ab136808

2. Antibodies tested in In-Cell ELISA

Many of our monoclonal antibodies are suitable for use in In-Cell ELISA and can be purchased separately.

View below our monoclonal antibodies tested in In-Cell ELISA.

NameReacts withMitoSciences codeAbcam code

ACAA1

Hu, Ms, Rat

MS716

ab110289

ACAT1

Hu

MS718

ab110290

ACADM

Hu, Ms, Rat, Cow

MS726

ab110296

ACADS

Hu

MS784

ab110318

Aconitase 2

Hu, Ms, Rat

MS786

ab110320

Aconitase 2

Hu, Ms, Rat

MS793

ab110321

AGXT

Hu, Rat, Cow

MS773

ab110313

AIF

Hu

MSA09

ab110327

ALDH2

Hu, Ms, Rat, Cow

MS767

ab110311

Catalase

Hu, Rat, Cow

MS721

ab110292

Cleaved PARP

Hu

MS777

ab110315

CPT2

Hu, Ms, Rat, Cow

MS722

ab110293

CRYZ

Hu

MS766

ab110310

Cyclophilin F

Hu, Ms, Rat, Cow

MSA04

ab110324

Cytochrome C

Hu, Ms, Rat, Ce, Cow

MSA06

ab110325

DCXR

Hu

MS704

ab110283

DECR1

Hu

MS711

ab110287

ECH1

Hu, Rat

MS723

ab110294

Epoxide hydrolase

Hu

MS758

ab110307

FH

Hu, Rat, Cow

MS709

ab110286

GAPDH

Hu

MS751

ab110305

HADHA

Hu

MS702

ab110281

HADHB

Hu

MS733

ab110301

HADHSC

Hu

MS706

ab110284

HMGCL

Hu

MS725

ab110295

HSDL2

Hu

MS728

ab110298

Hsp60

Hu

MS772

ab110312

ETFA

Hu, Ms, Rat

MS782

ab110316

MDH2

Hu, Ms, Rat, Pig

MS783

ab110317

MGST3

Hu, Rat

MS765

ab110309

Mitochondrial Pyruvate dehydrogenase kinase 1

Hu, Cow

MSP49

ab110335

Mitofilin

Hu

MSM02

ab110329

Nitrotyrosine

Hu, Ms, Rat, Cow

MS703

ab110282

OGDH

Hu, Cow, Pig

MS753

ab110306

PCB

Hu, Ms, Rat, Cow

MS774

ab110314

Pyruvate Dehydrogenase E1-alpha subunit

Hu, Ms, Rat, Cow

MSP03

ab110330

Pyruvate Dehydrogenase E2

Hu, Cow

MSP05

ab110332

SIRT1

Hu, Ms, Rat

MS750

ab110304

Superoxide Dismutase 2

Hu, Ms, Rat, Cow

MS732

ab110300

VCP

Hu, Ms, Rat

MS764

ab110308

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