CytoPainter - the ideal platform for multicolor staining
CytoPainter is a multicolor staining platform that allows you to effectively visualize cellular components and cellular tracking.
- Simple method:
- prepare your cells, add the dye, incubate and analyze - Compatible with secondary antibodies and nuclear dyes
- for multicolor staining or GFP-transfected cells - Quick visualization of actin filaments, lysosomes and mitochondria
- ideal for co-localization studies - Label and track cells
- to study cellular motility and other cell events in a spatial and temporal context
Actin Filaments visible in 30 minutes
Actin filaments (also known as F-actin) are essential components of the cytoskeleton and are necessary for many cellular processes such as cell division, motility and cell signaling. Defects in F-actin formation that affect the cytoskeleton integrity are related to metastasis and neurodegenerative diseases such as Parkinson's.
Characteristics and advantages:
- Selective binding to F-actin - no cross-link with monomers or dimers
- Fluorescent phalloidin conjugate with high photostability - no photobleaching
- Four different colors available - ideal for co-localization experiments
- Suitable for fixed tissue sections or cell culture - easy to incorporate in your IF/ICC protocols
CytoPainter F-Actin Staining kits
| Color | Ex/Em (nm) | AbID | |
|---|---|---|---|
| Blue |  | 350/450 | ab112124 |
| Green |  | 500/520 | ab112125 |
| Orange |  | 550/575 | ab112126 |
| Red |  | 594/610 | ab112127 |
Visualization of mitochondria and lysosomes in live cells
Our unique proprietary fluorescent dyes are designed to selectively enter the specific subcellular organelles.
The lysotropic dye accumulates in the lysosome via the lysosome pH gradient (pH 4.5-4.8), while the mitochondrial membrane potential gradient is responsible for the accumulation of the mitochondrial dye. In both cases, live cells will retain the dye in the respective compartments, making these kits a powerful tool for spatial and temporal studies of these organelles.
Characteristics and advantages:
- Proprietary fluorescent dye with high photostability - no photobleaching
- Available in three to five different colors - ideal for co-localization experiments
- Suitable for live suspension and adherent cells (not for fixed cells)
CytoPainter Lysosomal Staining Kits
Conventional lysosomes, the organelles responsible for storing hydrolytic enzymes, are essential for dismantling and recycling intracellular substrates presented to them through endocytosis or phagocytosis, such as amino acids. Defects in lysosomal function are involved in important congenital syndromes such as Tay-Sachs or Nieman-Pick disease.
Our lysosomal staining kits enable co-localization studies of tagged proteins in live cells in conjunction with time-course experiments for lysosomal activity, and are an excellent alternative to LysoTracker® dyes.
| Color | Ex/Em (nm) | AbID | |
|---|---|---|---|
| Blue | | 360/445 | ab112135 |
| Green* | | 405/480 | ab112138 |
| Green | | 490/525 | ab112136 |
| Orange | | 540/560 | ab138895 |
| Red | | 540/590 | ab112137 |
| Deep red |  | 637/650 | ab138896 |
*Large Stokes shift - for multiplex fluorescence experiments
CytoPainter Mitochondrial Staining Kits
Being involved in a broad range of functions such as cell growth, cell cycle and apoptosis, mitochondria are central players in cellular activity. Defects on mitochondria are linked to several disorders, ranging from cancer to Alzheimer's
Our mitochondrial staining kits can be used in co-localization studies of tagged-proteins in live cells in conjunction with time-course experiments for mitochondrial activity, and are an excellent alternative to MitoTracker® dyes.
| Color | Ex/Em (nm) | AbID | |
|---|---|---|---|
| Green | | 490/520 | ab112143 |
| Orange* | | 405/575 | ab138897 |
| Orange | | 545/575 | ab112144 |
| Red | | 580/600 | ab112145 |
*Large Stokes shift - for multiplex fluorescence experiments
Visualize live cells for hours with no photobleaching
Analyze your cells of interest for hours thanks to our cell tracking dyes that are retained in living cells. The dyes are hydrophobic molecules that can permeate through the plasma membrane and become strongly fluorescent once inside the cell.
Characteristics and advantages:
- Proprietary fluorescent tracking dye with high photostability - no photobleaching
- Available in five different colors - ideal for co-localization studies with transfected cells
- Compatible with most common fluorescent microscope filters
- Suitable for proliferating and non-proliferating live suspension and adherent cells
CytoPainter Cell Tracking Kits
Cell tracking is an essential tool to study chemotaxis, cell adhesion or cell viability where it might be necessary to visualize the same set of cells through a prolonged exposure time, such as cells expressing a fluorescent-tagged protein.
| Color | Ex/Em (nm) | AbID | |
|---|---|---|---|
| Blue | | 360/445 | ab138890 |
| Green | | 490/525 | ab138891 |
| Orange | | 540/560 | ab138892 |
| Red | | 540/590 | ab138893 |
| Deep red | | 637/650 | ab138894 |
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