The excitation of the DyLight® 650 fluorophore is 652nm and the emission is 672nm. DyLight® 650 offers the same far red fluorescence and photostability as the DyLight® 649 fluorophore. The substitution of the DyLight® 649 dye will not require you to change your experimental set-up.
View frequently asked questions related to the substitution of DyLight® 649 by DyLight® 650.
DyLight® a new family of dyes
DyLight® fluorochromes are suitable for fluorescence microscopy, Western blotting (with fluorescent scanners) and flow cytometry.
- Bright fluorescence – intense emission provides superior sensitivity
- Highly photostable – better resistance to photobleaching
- Completely stable in buffers ranging from pH 4-9
Table: Spectral properties of DyLight® fluorochromes. In order to expand the spectra, please click on the spectrum icons.
Ex/Em: Excitation and emission wavelength in nanometers (+/- 4nm)
ε: Molar extinction coefficient at the absorption maximum
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Cy® and CyDye® are trademarks of GE Healthcare Limited. Alexa Fluor® is a trademark of Molecular Probes Inc.