The excitation of the DyLight® 650 fluorophore is 652nm and the emission is 672nm. DyLight® 650 offers the same far red fluorescence and photostability as the DyLight® 649 fluorophore. The substitution of the DyLight® 649 dye will not require you to change your experimental set-up.
View frequently asked questions related to the substitution of DyLight® 649 by DyLight® 650.
DyLight® a new family of dyes
DyLight® fluorochromes are suitable for fluorescence microscopy, Western blotting (with fluorescent scanners) and flow cytometry.
- Bright fluorescence – intense emission provides superior sensitivity
- Highly photostable – better resistance to photobleaching
- Completely stable in buffers ranging from pH 4-9
Table: Spectral properties of DyLight® fluorochromes. In order to expand the spectra, please click on the spectrum icons.
| Fluorochrome | Color | Ex/Em | Spectra | ε (M-1cm-1) | Spectrally equivalent dyes |
|---|---|---|---|---|---|
| DyLight® 488 |  | 493/518 |  | 70K | Alexa Fluor 488®, Fluorescein, FITC, Cy2® |
| DyLight® 550 |  | 562/576 |  | 150K | Alexa Fluor 555®, Cy3®, TRITC |
| DyLight® 594 |  | 593/618 |  | 80K | Alexa Fluor 594®, Texas Red® |
| DyLight® 650 |  | 652/672 |  | 250K | Alexa Fluor 647®, Cy5® |
Ex/Em: Excitation and emission wavelength in nanometers (+/- 4nm)
ε: Molar extinction coefficient at the absorption maximum
For further information or help, contact our Scientific Support team or have a look at frequently asked questions.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Cy® and CyDye® are trademarks of GE Healthcare Limited. Alexa Fluor® is a trademark of Molecular Probes Inc.
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