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Webinar Details
- Date: Wednesday, February 15, 2012
- Time: 09:00 PST, 12:00 EST, 17:00 GMT
- Duration: 60 minutes
- Presenter: Caitlin Buckspan, Scientific Support Specialist at Abcam, Inc.
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- Additional Western blot resources: A guide to Western blot Western blot protocol Western blot troubleshooting tips
Join Abcam for this live webinar with our Western Blot expert.
Western blot (WB), also called immunoblot, uses specific antibodies to analyze one protein in a sample containing multiple proteins. Proteins are separated from each other by size using gel electrophoresis, then transferred to a membrane and detected with a specific antibody. This technique is widely used in biological research, as it provides information about the molecular weight of a protein as well as relative differences in expression between samples.
Our resident WB expert will explain and answer your questions about:
- Sample preparation
- Electrophoresis
- Transfer of proteins and staining
- Controls
- Troubleshooting
- Antibody selection
About the presenter
Caitlin Buckspan received a B.S. in Biological Engineering from the University of Florida in 2007 and a M.S. in Bioengineering from the University of California, San Diego in 2010. At UF, she researched seasonal changes in termite hormone levels, and ran many Western blots. Her Master’s thesis focused on age-related differences in mesenchymal stem cells from mice. She joined the Abcam Scientific Support Team last year, and is a current member of the Histochemical Society and the International Society for Stem Cell Research.
Questions about this webinar?
Email events@abcam.com for the answers.