EpiSeeker: your Tool for advancing Epigenetics

Don't get lost in the maze of epigenetics

Our EpiSeeker kits are all you need to quantify and detect key epigenetic modifications.

Get to the best results in no time with Abcam's new range of epigenetic kits.

If you ever thought epigenetic experiments were complicated and laborious, think again!

Our EpiSeeker range has been developed with researchers in mind so you can spend more time designing your experiments and less time doing them.

Characteristics and advantages

• Simple and reliable assay
• Available in colorimetric / fluorometric format
• Stripwell microplate format: adaptable for manual or high throughput analysis

Need help preparing your nuclear extracts?

Our EpiSeeker range offers a selection of sample preparation kits that have been optimized to be used with the EpiSeeker detection kits.

For a complete list of sample preparation kits and more information, please visit our EpiSeeker Sample Preparation Guide.

Which epigenetic kit will you be using today?

We have a wide selection of epigenetic kits to detect and quantify:

• ChIP Kits
• DNA modification and methylation
• Histone demethylation
• Histone methylation activity
• Histone methylation quantification
• Histone acetylation and deacetylation
• Histone phosphorylation
• Sumoylation

ChIP Kits

ChIP (Chromatin Immunoprecipitation) is a versatile tool to understand how protein-chromatin interactions affect cellular processes such as gene transcription and gene silencing. Abcam ChIP kits offer simple and reliable assays to detect and quantify interactions in a broad range of situations.

Want to know more about ChIP? Check out our Beginner's Guide to ChIP for more information on the technique and other tips.

Immunoprecipitate methyl-histone H3 enriched DNA to study which proteins are involved in gene activation/ silencing.

Immunoprecipitate acetyl-histone H3 and H4 enriched DNA to study which proteins are involved in gene activation.

Methylation of cytosine residues located on CpG islands in promoter regions is generally associated with transcription repression. Hydroxymethylation (hydroxylation and methylation) of cytosine residues in promoter regions is a recently discovered modification in animal cells which seems to play a role in DNA demethylation, although its function is still unclear. Detect specific binding partners of these two modified residues with our kits.

Methyl-DNA binding protein 2 (MBD2) is an important player in "translating" DNA methylation signal into transcriptional repression. Determine MBD2 activity or its binding partners with our range of kits.

» back to top of the page

DNA modification and methylation

Check out our kits for detection and quantification of activity of DNA methyltranferases and demethyltransferases, the enzymes responsible for regulating DNA methylation at CpG dinucleotides. Have a look at our DNMT guide for more details on the products.

Bisulfite modification is used to determine the methylation pattern of DNA: DNA is treated with sulfites, a process which will convert cytosine residues to uracil while leaving 5-methylcytosine residues unaffected. Therefore, the bisulfite treatment will introduce specific changes in the DNA sequence depending on the methylation status of individual cytosine residues, producing single-nucleotide resolution information about the methylation status of the DNA sequence of interest.

Quantify methylated and hydroxymethylated cytosine residues for differentiated DNA methylation and demethylation patterns. 

» back to top of the page

Histone demethylation

Detect and quantify specific histone demethylase activity, whether you are interested in the LSD1 or the JmjC family of proteins.

» back to top of the page

Histone methylation activity

Quantify specific histone methyltransferase (HMT) activity in only 3 hours: quick, simple, and minimal hands-on work.

» back to top of the page

Histone methylation quantification

Histone H3 and H4 can be modified by addition of one, two, or three methyl groups on specific lysine residues at the N-terminal tail. The table below lists a selection of kits to measure the most popular methylation modifications, buta complete list of our methylated histone quantification products can be found here.

» back to top of the page

Histone acetylation and deacetylation

Histone H3 and H4 can be modified by addition on an acetyl group on specific lysine residues at the N-terminal tail, a modification generally linked to gene activation. Detect and quantify a variety of histone acetylation modifications with our kits.

» back to top of the page

Histone phosphorylation

Histone H3 can be modified by addition of phosphate group on specific serine residues at the N-terminal tail.

» back to top of the page

Sumoylation

Sumoylation is the covalent reversible addition of SUMO (Small Ubiquitin-like Modifier) proteins to other proteins in order to modify their function in cells (in a similar manner to ubiquitination). Detect and quantify total protein or specific HDAC1 sumoylation in your nuclear extracts.

» back to top of the page