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Tools For Measurement Of Protein Phosphorylation

ELISA and In-Cell ELISA kits

Post-translational modifications (PTM) regulate a wide range of cellular processes such as growth, cell cycle progession, differentiation, apoptosis, and neuronal communication.

Our catalog offers a comprehensive range of ELISA kits assessing phospho- and/or total protein levels:

ELISA kits

Conventional ELISAs are convenient and sensitive kits that monitor the activation or function of biological pathways in cell lysates.

Our ELISA kits are based on specific capture antibodies (anti-pan-antibody or anti-phosphorylated antibody) coated onto a 96-well plate.

Target 1

Target 2

Target 3

Species

Product code

Akt (pS473)

Total Akt

Hu, Ms, Rat

ab126433

Akt (pS473)

Hu, Ms, Rat

ab126432

EGFR (pS1070)

Hu

ab126434

EGFR (pS1070)

Total EGFR

Hu

ab126435

EGFR (pY1045)

Hu

ab126436

EGFR (pY1045)

Total EGFR

Hu

ab126437

EGFR (pY1068)

Hu

ab126438

EGFR (pY1068)

Total EGFR

Hu

ab126439

EGFR (pY1086)

Hu

ab126440

EGFR (pY1086)

Total EGFR

Hu

ab126441

EGFR (pY992)

Hu

ab126442

EGFR (pY992)

Total EGFR

Hu

ab126443

Erk1 (pT202/pY204)

Erk2 (pT185/pY187)

Hu, Ms, Rat

ab126444

Erk1 (pT202/pY204)

Erk2 (pT185/pY187)

Total ERK1/2

Hu, Ms, Rat

ab126445

Mek1 (pS217/221)

Hu, Ms, Rat

ab126446

Mek1 (pS217/221)

Total Mek1

Hu, Ms, Rat

ab126448

Met (pY1234/pY1235)

Hu, Ms

ab126450

Met (pY1234/pY1235)

Total Met

Hu, Ms

ab126451

p38α (pT180/pY182)

Hu, Ms

ab126452

p38α (pT180/pY182)

Total p38α

Hu, Ms

ab126453

p53

Hu

ab117995

p53 (pS46)

Hu

ab124532

p53 (pS392)

Hu

ab124533

PDHA1 MitoSciences

Hu, Ms, Rat, Cow

ab115342

PDHA1 (pS232) MitoSciences

Hu, Ms, Rat, Cow

ab115343

PDHA1 (pS293) MitoSciences

Hu, Ms, Rat, Cow

ab115344

PDHA1 (pS300) MitoSciences

Hu, Ms, Rat, Cow

ab115345

STAT1 (pS727)

Hu, Ms

ab126454

STAT1 (pS727)

Total STAT1

Hu, Ms

ab126455

STAT1 (pY701)

Hu, Ms

ab126456

STAT1 (pY701)

Total STAT1

Hu, Ms

ab126457

STAT3 (pY705)

Hu, Ms, Rat

ab126458

STAT3 (pY705)

Total STAT3

Hu, Ms, Rat

ab126459

In-Cell ELISA kits

In-Cell ELISA (ICE) kits allow the measurement of the relative amounts of protein phosphorylation and monitor the effect of various treatments, inhibitors or activators in cultured cell lines. The cells are then stimulated, fixed and blocked in the same well before incubating with phospho-specific antibodies.

Advantages

  • Simultaneously detect targets within the same microplate well and eliminate variability caused by differences in cell plating
  • No lysate preparation required
  • Assess the effect of various treatments in one experiment
  • Suitable for a wide range of cell lines
  • Alternative to Western blotting and sandwich ELISA

Target

Species

Product code

AKT (S473)

Hu, Ms

ab126578

AKT (S473) (Infrared)

Hu, Ms

ab126579

EGFR

Hu

ab126419

EGFR (Y1068)

Hu

ab126420

EGFR (Y845)

Hu, Ms, Rat

ab126421

EGFR (Y992)

Hu, Ms, Rat

ab126422

ERK1/2 (T202/Y204)

Hu, Ms, Rat

ab126423

JNK (T183/Y185)

Hu, Ms, Rat

ab126424

p38 MAPK (T180/Y182)

Hu, Ms, Rat

ab126425

p53 (S46)

Hu

ab128570

p53 (S392)

Hu

ab128571

PDH

MitoSciences

Hu

ab110219

PDH (Infrared)

MitoSciences

Hu

ab110218

Phosphotyrosine

Hu, Ms, Rat

ab126431

STAT1 (Y701)

Hu, Ms

ab126426

STAT3 (Y705)

Hu, Ms, Rat

ab126427

STAT4 (Y693)

Hu, Ms

ab126428

STAT5 (Y694)

Hu

ab126429

STAT6 (Y641)

Hu, Ms

ab126430

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