- Date: June 21, 2012
- Time: 17:00 BST, 12:00 EDT, 09:00 PDT
- Duration: 60 minutes
- Language: English
John Constable, Abcam
In-Cell ELISA, is a well-accepted assay approach to perform quantitative immunocytochemistry of cultured cells using either a colormetric or fluorometric readout. The technique generates quantitative data with specificity similar to western blotting, but with much greater quantitative precision and higher throughput due to the greater dynamic range and linearity of direct fluorescence detection and the ability to run 96 samples in parallel. This method rapidly fixes the cells in situ, stabilizing the in vivo levels of proteins, and thus essentially eliminates changes during sample handling, such as preparation of protein extracts.
The webinar topics will include:
- Introduction - why use an ICE assay
- Benefits of In-Cell Technology
- Key reagents
- Optimization and Troubleshooting
Online registration is now open. Register and attend this FREE webinar.
About the presenter
John Constable has a PhD in Physiology from the University of Liverpool, United Kingdom and six years postdoctoral experience at the University of Oregon. His research focused on the development and function of the neuronal synapse, he spent his graduate studies looking into exocystosis and his post-doctoral years studying the process of synaptogenesis. He has used a variety of western blotting, immnuofluorescence and ELISA based applications to study these processes.
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