During the onset of apoptosis, phospholipid asymmetry on the plasma membrane changes resulting in exposure of phosphatidylserine (PS) residues.
Kinetic Apoptosis kits detect both transient and permanent phosphatidylserine (PS) exposure to quantify cell death as it is taking place.
Features and benefits:
- No washing required - you won't lose any precious cells
- Minimal background fluorescence - no more struggling with FACS or filter compensation
- Suitable for live-cell imaging - follow the changes on a single cell basis
How do our kinetic apoptosis kits work?
Abcam's Kinetic Apoptosis Kits contain pSIVA™-IANBD, a probe that attaches to exposed PS but detaches if PS is flipped back. This feature allows researchers to study a whole range of events such as:
- Viability/ cell death upon drug exposure
- Tissue development-related apoptosis
- Apoptotic events in real time
- Normal physiological events when membrane is flipped: vesicle release or lymphocyte activation
Apoptosis kits comparison
| AnnexinV-FITC | Kinetic Appoptosis Kit (Flow Cytometry) | Kinetic Apoptosis Kit (Microscopy) | |
|---|---|---|---|
| Product code | ab14085 | ab129816 | ab129817 |
| Probe | annexin V | pSIVA-IANBD (annexin XII) | pSIVA-IANBD (annexin XII) |
| Number of tests | 100 | 25 / 100 | 10 - 20 |
| Detection method | flow cytometry/ microscopy | flow cytometry | microscopy |
| Early apoptosis detection | ✔ | ✔ | ✔ |
| Viability assessment | ✔ | ✔ | ✔ |
| Toxicity assessment | ✔ | ✔ | ✔ |
| Fluorescence only when bound to PS | ✔ | ✔ | |
| Transient PS exposure detection | ✔ | ||
| Live-cell imaging | ✔ | ||
| in vivo imaging | ✔ | ✔ | |
| High-throughput screening | ✔ | ✔ |
