Kinetic Apoptosis Kits

Detect apoptosis in real time

Phosphatidylserine (PS) exposure measurement 

During the onset of apoptosis, phospholipid asymmetry on the plasma membrane changes resulting in exposure of phosphatidylserine (PS) residues.

Kinetic Apoptosis kits detect both transient and permanent phosphatidylserine (PS) exposure to quantify cell death as it is taking place.

 

Features and benefits:

  • No washing required - you won't lose any precious cells
  • Minimal background fluorescence - no more struggling with FACS or filter compensation
  • Suitable for live-cell imaging - follow the changes on a single cell basis

 

How do our kinetic apoptosis kits work?

Abcam's Kinetic Apoptosis Kits contain pSIVA™-IANBD, a probe that attaches to exposed PS but detaches if PS is flipped back. This feature allows researchers to study a whole range of events such as:

  • Viability/ cell death upon drug exposure
  • Tissue development-related apoptosis
  • Apoptotic events in real time
  • Normal physiological events when membrane is flipped: vesicle release or lymphocyte activation

Phosphatidylserine (PS) exposure mechanism in apoptotic cells



Apoptosis kits comparison

 AnnexinV-FITCKinetic Appoptosis Kit  (Flow Cytometry)Kinetic Apoptosis Kit (Microscopy)
Product code ab14085 ab129816 ab129817
Probe annexin V pSIVA-IANBD (annexin XII) pSIVA-IANBD (annexin XII)
Number of tests

 100

 25 / 100

 10 - 20

Detection method  flow cytometry/ microscopy  flow cytometry  microscopy
Early apoptosis detection

Viability assessment
Toxicity assessment
Fluorescence only when bound to PS  
Transient PS exposure detection    
Live-cell imaging    
in vivo imaging  
High-throughput screening  

 

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