VeriBlot | your choice for the WB detection of IP samples

VeriBlot for IP products enable the trouble-free detection of immunoprecipitated proteins, without interference of heavy and light-chains

Using secondary antibodies, recognizing the heavy and light-chain of the primary antibody for WB detection of IP samples will always result in two bands (the heavy-chain at 50kDa and the light-chain at 25kDa).

These bands are due to the secondary antibody binding to the primary antibody released with the antigen during the immunoprecipitation procedure, or to the recognition of endogenous IgGs from the lysate itself.


Problem:

  • Protein of interest is ~50kDa - heavy-chain will obstruct its detection
  • Protein of interest is ~25kDa - light-chain will obstruct its detection

Solution:


Download our Veriblot for IP flyer
Heavy and light chain versus light chain
  • Lane 1: 0.5μg of mouse monoclonal    
  • Lane 2: 0.25μg of mouse monoclonal  
  • Lane 3: 0.125μg of mouse monoclonal

VeriBlot for IP (for IP products <30kDa and co-immunoprecipitation experiments)


Product name

Conjugate

Product code

Anti-goat IgG VeriBlot for IP secondary antibody

HRP

ab157532

Anti-mouse IgG VeriBlot for IP secondary antibody

Biotin

ab131367

Anti-mouse IgG VeriBlot for IP secondary antibody

HRP

ab131368

Anti-rabbit IgG VeriBlot for IP secondary antibody

AP

ab131365

Anti-rabbit IgG VeriBlot for IP secondary antibody

HRP

ab131366

Tip: Please make sure to completely reduce the immunoprecipitate and to use milk as a blocking agent.


Light-chain specific secondary antibodies (for IP products >30kDa)


AP conjugated light-chain specific secondary antibodies

Product name

Applications

Product code

Mouse monoclonal [JDC-12] to Human lambda - light chain

ELISA, Dot Blot, WB, ICC, IHC-Fr

ab99810

Rat monoclonal [187.1] to Mouse kappa - light chain

Flow Cyt, ELISA, ICC/IF, WB

ab99616

Rat monoclonal [JC5-1] to Mouse lambda - light chain

Flow Cyt, ICC/IF, ELISA

ab99624

Rat monoclonal [H139-52.1] to Mouse kappa - light chain

Flow Cyt, ICC/IF, WB, ELISA

ab99631

Mouse monoclonal [SB62a] to Rabbit IgG - light chain

ELISA, WB

ab99696

Mouse monoclonal [K4F5] to Rat kappa - light chain

ELISA, WB

ab99691


HRP conjugated light-chain specific secondary antibodies

Product name

Applications

Product code

Mouse monoclonal [JDC-12] to Human lambda - light chain

ELISA, Dot Blot, WB, ICC, IHC-Fr

ab99811

Rat monoclonal [187.1] to Mouse kappa - light chain

ELISA, ICC/IF, WB

ab99617

Rat monoclonal [JC5-1] to Mouse lambda - light chain

ICC/IF, ELISA

ab99625

Rat monoclonal [H139-52.1] to Mouse kappa - light chain

ICC/IF, WB, ELISA

ab99632

Mouse monoclonal [SB62a] to Rabbit IgG - light chain

ELISA, WB

ab99697

Mouse monoclonal [K4F5] to Rat kappa - light chain

ELISA, WB

ab99692


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