The ability to produce a homogenous population of functional cells in large quantities is one of the major challenges for stem cell biology. This is a robust 17 day differentiation protocol to direct human embryonic stem cells (hESCs) towards hepatocyte-like cells.
It contains detailed information about:
- Preparation of chemical stocks
- Coating of culture plasticware
- Preparation of media
- Priming hESCs to definitive endoderm
Edited protocol reproduced with kind permission of David Hay and the Journal of Visualized Experiments
Download the differentiation protocol.
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