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Alexa Fluor® conjugated secondary antibodies

Alexa Fluor®

Our Alexa Fluor® conjugated secondary antibodies are extensively tested to help ensure bright staining and low background. We offer:

  • Why choose Alexa Fluor® conjugated secondaries?

  • Tested extensively in our laboratories
    – guarantee bright staining and low background
  • Large range of pre-adsorbed antibodies
    – ensure low species cross-reactivity
  • Dilution range of 1/200 -1/1000 (10-2 µg/ml) in IF/ICC
    – perform at least 250 stainings*
  • Antibodies conjugated to Alexa Fluor®405/488/555/568/594/647/750
    – for your multi-color imaging experiments

Emission spectra of Alexa Fluors®

Alexa Fluor® dye

Absorption max (nm)

Emission max (nm)

Extinction coefficient1

Quantum yield2

  • Alexa Fluor® 405
  • Alexa Fluor® 488
  • Alexa Fluor® 555
  • Alexa Fluor® 568
  • Alexa Fluor® 594
  • Alexa Fluor® 647
  • Alexa Fluor® 750
  • 402
  • 495
  • 555
  • 578
  • 590
  • 650
  • 749
  • 421
  • 519
  • 565
  • 603
  • 617
  • 668
  • 775
  • 35,000
  • 73,000
  • 155,000
  • 88,000
  • 92,000
  • 270,000
  • 290,000
  • -
  • 0.92
  • 0.1
  • 0.69
  • 0.66
  • 0.33
  • 0.12
*assuming a 1/200 dilution in 200 μl PBS
1 Efficiency of excitation light absorption. The higher the excitation coefficient the higher the likelihood of light absorption in a certain wavelength region.
2 Ratio of number of photons emitted to the number of photons absorbed – also known as yield of emitted light. The closer to 1 the quantum yield of a fluorochrome is the more efficient is the fluorescence process.
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.
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