Recombinant Anti-NeuroD1 antibody [EPR4008] (ab109224)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4008] to NeuroD1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-NeuroD1 antibody [EPR4008]
See all NeuroD1 primary antibodies -
Description
Rabbit monoclonal [EPR4008] to NeuroD1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Y79 and fetal brain lysates; ICC/IF: Mouse primary neuron cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4008 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
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Notes |
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ICC/IF
1/200. |
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa). |
Target
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Function
Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box. -
Involvement in disease
Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4760 Human
- Entrez Gene: 18012 Mouse
- Entrez Gene: 29458 Rat
- Omim: 601724 Human
- SwissProt: Q13562 Human
- SwissProt: Q60867 Mouse
- SwissProt: Q64289 Rat
- Unigene: 574626 Human
see all -
Alternative names
- atonal antibody
- basic helix loop helix transcription factor antibody
- BETA 2 antibody
see all
Images
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All lanes : Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution
Lane 1 : Y79 lysates
Lane 2 : Fetal brain lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling NeuroD1 with ab109224 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in a little of mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab109224 has been referenced in 13 publications.
- Matsui S et al. High mRNA expression of POU2F3 in small cell lung cancer cell lines predicts the effect of lurbinectedin. Thorac Cancer 13:1184-1192 (2022). PubMed: 35278040
- Olsen RR et al. ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer. Genes Dev 35:847-869 (2021). PubMed: 34016693
- Wang LL et al. Revisiting astrocyte to neuron conversion with lineage tracing in vivo. Cell 184:5465-5481.e16 (2021). PubMed: 34582787
- Kersigo J et al. Effects of Neurod1 Expression on Mouse and Human Schwannoma Cells. Laryngoscope 131:E259-E270 (2021). PubMed: 32438526
- Grunblatt E et al. MYCN drives chemoresistance in small cell lung cancer while USP7 inhibition can restore chemosensitivity. Genes Dev 34:1210-1226 (2020). PubMed: 32820040