alpha Tubulin antibody (ab18251)

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Anonymous Abreview submitted on 26 October 2006
Application:	Immunocytochemistry/ Immunofluorescence
alpha Tubulin antibody (ab18251)
Sample	Fruit fly (Drosophila melanogaster) Cell (embryos)
Specification	embryos
Fixative:	Methanol
Blocking step:	BSA as blocking agent for  1 hour  · Concentration: 2
Primary Antibody
Dilution	1/200
Incubation time:	1 hour
Secondary Antibody
Name:	Non-Abcam Antibody was used: alexa 488 goat anti-rabbit Conjugation: Alexa Fluor® 488
Dilution:	1/150
Additional Data
Rating	Good
Additional notes	Fixation in methanol 20 minutes,wash in PBS 3 times for 15 minutes The image represents a Drosophila embryo stained for tubulin with alpha tubulin 18251 from Abcam and Alexa 488 (green) , actin (red with Alexa 568 Rhodamine phalloidin) and TOTO -3 iodide for DNA (blue)
Image	alpha Tubulin antibody for ICC/IF (Fruit fly (Drosophila melanogaster)) Copyright image used under license

review by Kirk McManus submitted on 27 February 2006
Application:	Immunocytochemistry/ Immunofluorescence
alpha Tubulin antibody (ab18251)
Sample	Human Cell (HeLa)
Specification	HeLa
Fixative:	Paraformaldehyde
Primary Antibody
Dilution	1/8000
Incubation time:	30 minutes
Secondary Antibody
Name:	Non-Abcam Antibody was used: Goat Anti-Rabbit IgG (H+L) Conjugation: Cy3 ®
Dilution:	1/200
Additional Data
Rating	Excellent
Additional notes	I also used this antibody for a Western Blot with a whole cell extract from HeLa and HCT116 cells at 1:10,000 and it worked extremely well. It could probably be diluted to 1:20,000 and still get very strong signal. I have also used this antibody to immunofluorescently label Indian muntjac (IM) cells. It worked extremely well at 1:10,000. The image is an interphase IM cell labeled with anti-alpha tubulin.
Image	alpha Tubulin antibody for ICC/IF (Human) Copyright image used under license