MyD88 antibody (ab2064)

All antibodies work in different combinations of applications, species and samples, so we encourage our customers to write a short Abreview, so that future customers can make an informed decision about the usefulness of a particular antibody for their specific experiments.

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Anonymous Abreview submitted on 21 April 2009
Application:	Western blot
MyD88 antibody (ab2064)
Sample	Rat Cell lysate - whole cell (smooth muscle cells)
Loading amount:	15 µg
Specification	smooth muscle cells
Gel Running Conditions:	Reduced Denaturing (10%)
Blocking step:	Milk as blocking agent for  1 hour  · Concentration: 5 % · Temperature: 22°C
Primary Antibody
Dilution	1/1000
Incubation time:	15 hours  Temperature: 4°C Diluent: 5% milk in T-TBS
Secondary Antibody
Name:	Non-Abcam Antibody was used: mouse anti-rabbit hrp antibody Conjugation: HRP
Dilution:	1/2000
Detection
Detection method:	ECL
Exposure:	1 minute and 0 seconds
Bands:	Specific: 35 kDa
Additional Data
Rating	Good
Additional notes	This antibody is good for Rat too.
Image	MyD88 antibody for WB (Rat) Copyright image used under license

Anonymous Abreview submitted on 12 January 2009
Application:	Western blot
MyD88 antibody (ab2064)
Sample	Mouse Cell lysate - whole cell (Endothelial cells)
Loading amount:	15 µg
Specification	Endothelial cells
Gel Running Conditions:	Reduced Denaturing (10%)
Blocking step:	Milk as blocking agent for  1 hour  · Concentration: 5 % · Temperature: 22°C
Primary Antibody
Dilution	1/1000
Incubation time:	15 hours  Temperature: 4°C Diluent: 5% milk T-TBS
Secondary Antibody
Name:	Non-Abcam Antibody was used: rabbit anti-mouse HRP Conjugation: HRP
Dilution:	1/2000
Detection
Detection method:	ECL
Exposure:	1 minute and 0 seconds
Bands:	Specific: 35 kDa
Additional Data
Rating	Excellent
Image	MyD88 antibody for WB (Mouse) Copyright image used under license

review by SAMATA TIWARI submitted on 4 July 2008
Application:	Western blot
MyD88 antibody (ab2064)
Sample	Mouse Cell lysate - whole cell (endothelial cells)
Loading amount:	30 µg
Specification	endothelial cells
Gel Running Conditions:	Reduced Denaturing
Blocking step:	Milk as blocking agent for  1 hour  · Concentration: 5 % · Temperature: 37°C
Primary Antibody
Dilution	1/10000
Incubation time:	16 hours  Temperature: 4°C Diluent: BSA 5%
Secondary Antibody
Name:	Abcam antibody: Rabbit IgG secondary antibody - H&L
Dilution:	1/5000
Detection
Detection method:	ECL+
Exposure:	10 minutes and 0 seconds
Bands:	Specific: 35 kDa
Positive Control:	beta actin
Additional Data
Rating	Excellent

Anonymous (click to contact this user) Abreview submitted on 17 April 2008
Application:	Western blot
MyD88 antibody (ab2064)
Sample	Mouse Tissue lysate - whole (Whole Lung Homogenate)
Loading amount:	20 µg
Specification	Whole Lung Homogenate
Gel Running Conditions:	Reduced Denaturing (12 %)
Blocking step:	BSA as blocking agent for  1 hour  · Concentration: 5 % · Temperature: RT°C
Primary Antibody
Dilution	1/500
Incubation time:	10 hours  Temperature: 4°C Diluent: 5 % BSA
Secondary Antibody
Name:	Non-Abcam Antibody was used: Goat anti Rabbit HRP Conjugation: HRP
Dilution:	1/2000
Detection
Detection method:	ECL
Exposure:	1 minute and 0 seconds
Bands:	Specific: ~36 kDa   Non-specific: NA kDa
Additional Data
Rating	Excellent
Image	MyD88 antibody for WB (Mouse) Copyright image used under license

review by Karppiah Muthumani submitted on 27 June 2007
Application:	Western blot
MyD88 antibody (ab2064)
Sample	Human Cell lysate - whole cell (PBMC)
Specification	PBMC
Treatment	HIV infected
Blocking step:	BSA as blocking agent for  1 hour  · Concentration: 5 % · Temperature: 25°C
Primary Antibody
Dilution	1/500
Incubation time:	2 hours  Temperature: 4°C Diluent: 2.5% BSA in PBS-T20
Secondary Antibody
Name:	Non-Abcam Antibody was used: Donkey anti-Rabbit IgG Conjugation: HRP
Detection
Detection method:	ECL
Exposure:	30 seconds
Bands:	Specific: 30-35 kDa
Additional Data
Rating	Excellent

Anonymous (click to contact this user) Abreview submitted on 1 November 2006
Application:	Western blot
MyD88 antibody (ab2064)
Sample	Human Recombinant protein (RAW 264.7 and 293 cells)
Loading amount:	15 µg
Specification	RAW 264.7 and 293 cells
Blocking step:	Milk as blocking agent for  1 hour  · Concentration: 4%
Primary Antibody
Dilution	1/1000
Incubation time:	2 hours
Secondary Antibody
Name:	Non-Abcam Antibody was used: Donkey anti rabbit Conjugation: HRP
Dilution:	1/50000
Detection
Detection method:	ECL
Exposure:	1 minute and 0 seconds
Bands:	Specific: ~33 kDa
Additional Data
Rating	Good
Additional notes	This antibody detects both overexpressed Human MyD88 as well as endogenous mouse MyD88 in RAW 264.7 cells. The image is a 1 min exposure, when exposed longer, the band from RAW cells becomes stronger and clearer. Lane 4 shows Flag-MyD88 overexpressed in 293 cells, 15 ug of total cell lysate was loaded.
Image	MyD88 antibody for WB (Human) Copyright image used under license

review by Lori Manzel submitted on 10 June 2004
Application:	Western blot
MyD88 antibody (ab2064)
Additional Data
Notes	This antibody worked wonderfully. I used it to detect full-length and dominant-negative MyD88 in primary airway epithelial cells. The dilutions I used were 1:750 for primary antibody and 1:10,000 secondary antibody. the detection system I used was ECL plus. I had tried using MyD88 antibodies from 2 other companies and could not get results.
Dilution notes	Diluted 1:750 in TTBS/5% non-fat milk. Incubated overnight at 4?C. Secondary antibody-anti rabbit HRP diluted 1:10,000.