Olig2 antibody (ab33427)

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review by Markus Kipp submitted on 19 January 2010
Application:	Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Olig2 antibody (ab33427)
Sample	Mouse Tissue sections (Brain)
Specification	Brain
Fixative:	Paraformaldehyde
Antigen retrieval step:	Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization:	No
Blocking step:	Serum as blocking agent for  1 hour  · Concentration: 1 % · Temperature: 18°C
Primary Antibody
Dilution	1/200
Incubation time:	18 hours  Temperature: 4°C
Secondary Antibody
Name:	Non-Abcam Antibody was used: Horse anti-rabbit IgG Conjugation: Biotin
Dilution:	1/50
Additional Data
Rating	Excellent
PubMed ID	PMID: 19799876: View
Image	Olig2 antibody for IHC-P (Mouse) Copyright image used under license

review by Marketa Valova submitted on 1 April 2009
Application:	Western blot
Olig2 antibody (ab33427)
Sample	Mouse Cell lysate - whole cell (differentiated adult neural stem cells)
Loading amount:	30 µg
Specification	differentiated adult neural stem cells
Gel Running Conditions:	Reduced Denaturing (10 %)
Blocking step:	Milk as blocking agent for  1 hour  · Concentration: 5 % · Temperature: 25°C
Primary Antibody
Dilution	1/800
Incubation time:	12 hours  Temperature: 8°C
Secondary Antibody
Name:	Non-Abcam Antibody was used: Anti-Rabbit igG - peroxidase Conjugation: HRP
Detection
Detection method:	ECL
Exposure:	2 minutes and 0 seconds
Bands:	Specific: 30 kDa
Additional Data
Rating	Good
Additional notes	whole cell lysate - differentiated (D8) adult neural stem cells in triplicate
Image	Olig2 antibody for WB (Mouse) Copyright image used under license

review by Marketa Valova submitted on 18 June 2009
Application:	Immunohistochemistry (PFA fixed)
Olig2 antibody (ab33427)
Sample	Mouse Tissue sections (mouse subventricular zone)
Specification	mouse subventricular zone
Primary Antibody
Dilution	1/1000
Incubation time:	12 hours  Temperature: 8°C
Secondary Antibody
Name:	Non-Abcam Antibody was used: Alexa Fluor 594 goat anti-rabbit IgG Conjugation: Alexa Fluor® 594
Dilution:	1/200
Additional Data
Rating	Good
Additional notes	Blue staining - DAPI
Image	Olig2 antibody for IHC (PFA fixed) (Mouse) Copyright image used under license

review by Ruma Raha-Chowdhury submitted on 21 August 2008
Application:	Western blot
Olig2 antibody (ab33427)
Sample	Rabbit Tissue lysate - whole (Brain, muscle)
Loading amount:	20 µg
Specification	Brain, muscle
Gel Running Conditions:	Reduced Denaturing (12)
Blocking step:	Milk as blocking agent for  1 hour  · Concentration: 5 % · Temperature: RT°C
Primary Antibody
Dilution	1/500
Incubation time:	24 hours  Temperature: 4°C
Secondary Antibody
Name:	Non-Abcam Antibody was used: DAKO cytomation, Polyclonal Swine antirabbit immun Conjugation: HRP
Dilution:	1/3000
Detection
Detection method:	ECL+
Exposure:	1 hour, 0 minutes and 0 seconds
Bands:	Specific: 32 kDa   Non-specific: ~ 52, 54, ~22 kDa
Positive Control:	Mouse brain lysate
Negative Control:	Muscle lysate
Additional Data
Rating	Excellent
Additional notes	Excellent antibody, very clean. although it shows two or three band pattern, this is a charecteristic of Olig2 protein. Lane 1: muscle, Lane 2: brain, Lane 3: injured brain after 24 hours, Lane 4: injured brain after 7 days. All samples were prepared from mouse tissues.
Image	Olig2 antibody for WB (Rabbit) Copyright image used under license

Anonymous (click to contact this user) Abreview submitted on 4 August 2008
Application:	Immunocytochemistry/ Immunofluorescence
Olig2 antibody (ab33427)
Sample	Mouse Cell (neural stem cell)
Specification	neural stem cell
Fixative:	Paraformaldehyde
Permeabilization:	Yes - Triton X100
Blocking step:	Serum as blocking agent for  1 hour  · Concentration: 3 % · Temperature: rt°C
Primary Antibody
Dilution	1/2000
Incubation time:	16 hours  Temperature: 4°C
Secondary Antibody
Name:	Non-Abcam Antibody was used: Invitrogen Conjugation: Alexa Fluor® 488
Dilution:	1/1000
Additional Data
Rating	Excellent
Image	Olig2 antibody for ICC/IF (Mouse) Copyright image used under license

Anonymous (click to contact this user) Abreview submitted on 10 July 2008
Application:	Immunohistochemistry (Frozen sections)
Olig2 antibody (ab33427)
Sample	Mouse Tissue sections (E14.5 mouse brain)
Specification	E14.5 mouse brain
Fixative:	Paraformaldehyde
Permeabilization:	Yes - 0.1% Triton X-100
Blocking step:	Serum as blocking agent for  30 mins  · Concentration: 10 % · Temperature: 20°C
Primary Antibody
Dilution	1/500
Incubation time:	16 hours  Temperature: 4°C Diluent: 1% normal donkey serum
Secondary Antibody
Name:	Non-Abcam Antibody was used: cy3-conjugated donkey anti-rabbit IgG Conjugation: Cy3 ®
Dilution:	1/200
Additional Data
Rating	Good
Image	Olig2 antibody for IHC-Fr (Mouse) Copyright image used under license

Anonymous Abreview submitted on 13 October 2009
Application:	Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Olig2 antibody (ab33427)
Sample	Mouse Tissue sections (Mouse forebrain E17)
Specification	Mouse forebrain E17
Fixative:	Paraformaldehyde
Antigen retrieval step:	Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization:	Yes - 0.025% TX100
Blocking step:	Serum as blocking agent for  2 hours  · Concentration: 10 % · Temperature: 21°C
Primary Antibody
Dilution	1/500
Incubation time:	18 hours  Temperature: 4°C Diluent: TX100 & PBS & 2% BSA
Secondary Antibody
Name:	Non-Abcam Antibody was used: Goat anti-Rabbit Biotin Conjugation: Biotin
Dilution:	1/1000
Additional Data
Rating	Excellent
Additional notes	ABC amplification. Ni-DAB visualisation. Ventricular zone labelling.
Image	Olig2 antibody for IHC-P (Mouse) Copyright image used under license
Abcam response	Permeabilization step is not a standard procedure on paraffin sections (IHC-P). We recommend doing a biotin block.

review by Piercesare Grimaldi submitted on 26 November 2007
Application:	Immunocytochemistry/ Immunofluorescence
Olig2 antibody (ab33427)
Sample	Mouse Cell (CNS, cerebellum)
Specification	CNS, cerebellum
Fixative:	Formaldehyde
Permeabilization:	No
Blocking step:	Serum as blocking agent for  12 hours  · Concentration: 1.5 % · Temperature: RT°C
Primary Antibody
Dilution	1/500
Incubation time:	12 hours  Temperature: 4°C Diluent: PBS
Secondary Antibody
Name:	Non-Abcam Antibody was used: Jackson Cy3 Conjugation: Cy3 ®
Additional Data
Rating	Below Average
Abcam response	I'm sorry this antibody is not working for you. Abcam recommends permeabilization to stain intracellular proteins, and fixation with paraformaldehyde instead of formaldehyde. We also recommend increasing the primary concentration to 1/200.

review by Davy Yeung submitted on 26 June 2007
Application:	Immunocytochemistry/ Immunofluorescence
Olig2 antibody (ab33427)
Sample	Rat Cell (CG4 cell line (OPC))
Specification	CG4 cell line (OPC)
Fixative:	Paraformaldehyde
Permeabilization:	Yes - 0.1% Triton X
Blocking step:	Serum as blocking agent for  30 mins  · Concentration: 5 % · Temperature: 25°C
Primary Antibody
Dilution	1/200
Incubation time:	2 hours  Temperature: 25°C Diluent: PBS
Secondary Antibody
Name:	Non-Abcam Antibody was used: Alexa Goat anti Rabbit Conjugation: Alexa Fluor® 488
Dilution:	1/200
Additional Data
Rating	Good
Image	Olig2 antibody for ICC/IF (Rat) Copyright image used under license

Anonymous Abreview submitted on 1 May 2007
Application:	Immunohistochemistry (Frozen sections)
Olig2 antibody (ab33427)
Sample	Rat Tissue sections (Brain)
Specification	Brain
Fixative:	Paraformaldehyde
Blocking step:	Serum as blocking agent for  1 hour  · Concentration: 5%
Primary Antibody
Dilution	1/500
Incubation time:	18 hours
Secondary Antibody
Name:	Non-Abcam Antibody was used: AlexaFluor 488 goat anti rabbit IgG Conjugation: Alexa Fluor® 488
Dilution:	1/400
Additional Data
Rating	Good
Additional notes	4% PFA fixation Antigen retrieval was done with 1% SDS for 5 min at room temperature.
Image	Olig2 antibody for IHC-Fr (Rat) Copyright image used under license