Anti-4 Hydroxynonenal antibody (ab46545)
Key features and details
- Rabbit polyclonal to 4 Hydroxynonenal
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-4 Hydroxynonenal antibody
See all 4 Hydroxynonenal primary antibodies -
Description
Rabbit polyclonal to 4 Hydroxynonenal -
Host species
Rabbit -
Specificity
Specifically binds to HNE modified proteins. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 4 Hydroxynonenal.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading...
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Purification notes
This antibody was purified by an HNE modified Protein-Sepharose affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (11) |
1/1000.
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Notes |
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WB
1/1000. |
Target
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Relevance
Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress. -
Cellular localization
Cytoplasmic -
Alternative names
- 4 HNE antibody
- 4-Hydroxy-2-Nonenal antibody
Images
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All lanes : Left: ab46545 at 1/1000 dilution
Right: ab48506
Lane 1 : BSA cell lysate at 0.5 µg
Lane 2 : BSA cell lysate at 1 µg
Lane 3 : 4-Hydroxynonenal (BSA) cell lysate at 0.5 µg
Lane 4 : 4-Hydroxynonenal (BSA) cell lysate at 1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 kDa why is the actual band size different from the predicted?Western blot: Anti-4-HNE antibody (ab46545) staining at 1/1000 dilution, shown in black. In Western blot, ab46545 binds to 4-HNE but shows some non-specific binding to BSA. We recommend ab48506 for Western blot of 4-HNE. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 3 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) at 1/50000 dilution.
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Frozen mouse cardiac tissue was homogenized with lysis buffer containing 50 mmol/L Tris-HCl (pH7.5), 5 mmol/L EDTA, 10 mmol/L EGTA, 1X cock tail protease inhibitor, 1X alkaline phosphatase inhibitor and 1X acid phophatase inhibitor, 50 ug/ml phenylmenthysulfonyl fluoride and 1.23 mg/ml Chaps. Extracts were centrifuged at 12,000 rpm at 4°C for 15 minutes. 10 ug of the sample proteins was mixed with loading buffer (40 mmol/L Tris-HCl, pH 6.8, 1% SDS, 50 mmol/L DTT, 7.5% glycerol and 0.003% bromophenol blue and heated at 95°C for 5 minutes, and subjected to electrophoresis on a gradient gel (4% to 12%) at 120V. After electrophoresis, the protein was transferred to a PVDF membrane in a transfer buffer. The PVDF membrane was rinsed briefly in TBS buffer containing 50 mM Tris, 137 mM NaCl, pH 7.5 and blocked in buffer (5% milk with 0.5% BSA in TBST buffer (TBS buffer containing 0.1% tween 20) at room temperature for 1 hour. The membrane was then incubated with rabbit anti 4-hydroxy-2-noneal (4HNE) antibody at 1/3000 dilution at 4°C over night, followed by washing three times. The secondary antibody was incubated with the membrane for another one hour at room temperature. Finally the antigen-antibody complexes were visualized with use of an enhanced chemiluminescence kit. Anti-GAPDH (Abcam) was used for normalizing.
Datasheets and documents
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SDS download
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Datasheet download
References (529)
ab46545 has been referenced in 529 publications.
- Duan J et al. ASK1 inhibitor NQDI‑1 decreases oxidative stress and neuroapoptosis via the ASK1/p38 and JNK signaling pathway in early brain injury after subarachnoid hemorrhage in rats. Mol Med Rep 27:N/A (2023). PubMed: 36633130
- Guo Y et al. Hyperglycemia Induces Meibomian Gland Dysfunction. Invest Ophthalmol Vis Sci 63:30 (2022). PubMed: 35072689
- Tan W et al. Wnt inhibitory 1 ameliorates neovascularization and attenuates photoreceptor injury in an oxygen-induced retinopathy mouse model. Biofactors 48:683-698 (2022). PubMed: 35080047
- Zhong FY et al. The Role of CD147 in Pathological Cardiac Hypertrophy Is Regulated by Glycosylation. Oxid Med Cell Longev 2022:6603296 (2022). PubMed: 35096272
- Martin D et al. Oxidative and glycolytic skeletal muscles deploy protective mechanisms to avoid atrophy under pathophysiological iron overload. J Cachexia Sarcopenia Muscle 13:1250-1261 (2022). PubMed: 35118832