Blooms Syndrome Protein Blm antibody (ab476)

All antibodies work in different combinations of applications, species and samples, so we encourage our customers to write a short Abreview, so that future customers can make an informed decision about the usefulness of a particular antibody for their specific experiments.

Submit an Abreview and earn Abpoints

review by Carolyn McNees submitted on 30 July 2003
Application:	Immunofluorescence
Blooms Syndrome Protein Blm antibody (ab476)
Additional Data
Notes	The antibody worked beautifully for immunofluorescence using paraformaldehyde/Triton-X-100 fixation and an antibody dilution of 1:600 overnight at 4 degrees.
Dilution notes	Immunofluorescence 1:600

review by Jaana Jurvansuu submitted on 16 July 2003
Application:	Immunofluorescence
Blooms Syndrome Protein Blm antibody (ab476)
Additional Data
Notes	The BLM antibody worked nicely in immunofluorescence. In western blot the BLM protein was also well visible although there was quite a lot of background.
Dilution notes	1:500 for immunofluorescence 1:2000 for Western blot
Abcam response	Thank you for your very useful feedback information. We would suggest testing out different blocking agents, and/or decreasing the concentration of the primary antibody.

review by Adam Rosendorff submitted on 11 October 2002
Application:	Western blot
Blooms Syndrome Protein Blm antibody (ab476)
Additional Data
Notes	The Blm antibody worked well for western blotting, where a number of background bands were also visible, with a whole cell lysate. However immunoprecipitation followed by western blotting revealed a single band.
Dilution notes	1:400 (WB) .

review by Clare Fasching submitted on 13 September 2002
Application:	Immunofluorescence
Blooms Syndrome Protein Blm antibody (ab476)
Additional Data
Notes	I used the Rb polyclonal to BLM (Bloom's syndrome protein) for immunofluorescence(1:500). This protocol worked great with a methanol fixation/permeablization, but not with a paraformaldehyde fixation and a triton X-100 permeablization. This antibody also worked well for my westerns (1:1000), although there was an extremely high amount of background bands. I wouldn't have been able to determine the BLM band without a negative control.
Dilution notes	Immunofluorescence 1:500 Western 1:1000 .

review by Renjie Jiao submitted on 11 July 2001
Application:	Western blot
Blooms Syndrome Protein Blm antibody (ab476)
Additional Data
Notes	ab476 is fine for doing western blot and immunostaining on culture cells. It was not successful to use ab476 to do coimmunoprecipitation.

Anonymous Abreview submitted on 1 March 2001
Application:	Immunofluorescence
Blooms Syndrome Protein Blm antibody (ab476)
Additional Data
Notes	This antibody works fine as well on mouse embryos by whole-mount immunohistochemistry. At 1:100 dilution, still you can get nice staining.