Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)

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Anonymous (click to contact this user) Abreview submitted on 22 February 2008
Application:	Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end (ab52971)
Sample	Human Tissue sections (Human breast cancer metastasis)
Specification	Human breast cancer metastasis
Fixative:	Formaldehyde
Antigen retrieval step:	Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization:	No
Primary Antibody
Dilution	1/100
Incubation time:	18 hours  Temperature: 4°C Diluent: TNB
Secondary Antibody
Name:	Non-Abcam Antibody was used: Vector Goat anti-Rabbit HRP Conjugation: HRP
Dilution:	1/500
Additional Data
Rating	Good
Additional notes	This antibody would not work for me in a simple two-step fluorescent protocol. Appears rather sensitive to overfixation. It works well, however, with tyramide signal amplification or standard DAB techniques. Here, I've used the Perkin Elmer TSA-Cy3 kit. The image demonstrates a cancer metastasis at 40x with beta1 staining (in red) in both blood vessels and tumour cells. Blue is Hoechst for nuclei.
Image	Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end for IHC-P (Human) Copyright image used under license