FANCD2 antibody [FI-17] - ChIP Grade (ab5360)

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review by Wei-Ting Lu submitted on 9 May 2007
Application:	Western blot
FANCD2 antibody [FI-17] - ChIP Grade (ab5360)
Sample	Human Cell lysate - whole cell (thp1 cells)
Loading amount:	150 µg
Specification	thp1 cells
Blocking step:	BSA as blocking agent for  1 hour  · Concentration: 3%
Primary Antibody
Dilution	1/1000
Incubation time:	18 hours
Secondary Antibody
Name:	Non-Abcam Antibody was used: ECL Mouse IgG, HRP-Linked (from sheep) Conjugation: HRP
Dilution:	1/3000
Detection
Detection method:	ECL
Exposure:	32 minutes and 0 seconds
Bands:	Specific: ~155 kDa   Non-specific: 100 kDa
Additional Data
Rating	Average
Additional notes	This antibody works better in IP than in WB application.
Image	FANCD2 antibody [FI-17] - ChIP Grade for WB (Human) Copyright image used under license

Anonymous (click to contact this user) Abreview submitted on 9 May 2007
Application:	Immunoprecipitation
FANCD2 antibody [FI-17] - ChIP Grade (ab5360)
Sample	Human Cell lysate - whole cell (THP1)
Total protein in input:	1000 µg
Specification	THP1
Immuno- precipitation step	Protein A/G
Primary Antibody
Concentration	4 µg/mg lysate
Incubation time:	18 hours
Western Blot Antibody
Name:	Abcam antibody: Rabbit IgG secondary antibody - H&L
Dilution:	1/5000
Additional Data
Rating	Good
Additional notes	Sample immunoprecipitated with ab5360, 4ug antibody versus 1mg cell lysate. Primary antibody = rabbit anti-FANCD2 antibody, Novus Biological FANCD2-S and -L were clearly seen in the figure. Left lane is non-irradiated samples while right lane is sample irradiated with 5 Gray (Gy) ionizing irradiation. FANCD2-L is seen in non-irradiated sample due to S-phase progression.
Image	FANCD2 antibody [FI-17] - ChIP Grade for IP (Human) Copyright image used under license