F4/80 antibody [CI:A3-1] (ab6640)

All antibodies work in different combinations of applications, species and samples, so we encourage our customers to write a short Abreview, so that future customers can make an informed decision about the usefulness of a particular antibody for their specific experiments.

Submit an Abreview and earn Abpoints

review by Ling Guo submitted on 10 July 2008
Application:	Immunohistochemistry (Frozen sections)
F4/80 antibody [CI:A3-1] (ab6640)
Sample	Mouse Cell (Mouse Spleen)
Specification	Mouse Spleen
Fixative:	Acetone
Permeabilization:	No
Blocking step:	BSA as blocking agent for  30 mins  · Concentration: 5 % · Temperature: 25°C
Primary Antibody
Dilution	1/100
Incubation time:	12 hours  Temperature: 4°C Diluent: 1% BSA in PBS
Secondary Antibody
Name:	Non-Abcam Antibody was used: Jackson ImmunoResearch, Goat anti-rat Conjugation: Rhodamine
Dilution:	1/200
Additional Data
Rating	Excellent
Image	F4/80 antibody [CI:A3-1] for IHC-Fr (Mouse) Copyright image used under license

review by Andreas Gille submitted on 16 November 2007
Application:	Immunohistochemistry (Frozen sections)
F4/80 antibody [CI:A3-1] (ab6640)
Sample	Mouse Tissue sections (liver, spleen)
Specification	liver, spleen
Fixative:	Acetone
Permeabilization:	Yes - AB dilution contained 0.1% TX-100
Blocking step:	Serum as blocking agent for  30 mins  · Concentration: 10 % · Temperature: 25°C
Primary Antibody
Dilution	1/50
Incubation time:	24 hours  Temperature: 4°C Diluent: 10% goat serum in PBS with 0.1% TX-100
Secondary Antibody
Name:	Non-Abcam Antibody was used: Goat-anti rat Alexa-488 Conjugation: Alexa Fluor® 488
Dilution:	1/200
Additional Data
Rating	Good
Additional notes	Mice were perfused via heart with 10 ml PBS followed by 10 ml 4%PFA in PBS. Organs were left over night in 4% PFA/PBS at 4 C. Organs were washed in PBS and then transfered into a 30% succrose solution for cryoprotection and left over night at 4 C. Sections wer 10 um, dried and fixed in ice cold acetone for 10 min. Blocking was 30 min with 10 % normal goat serum. I have tried antibody dilutions of 1:50 to 1:400 and found 1:50 to be working best.
Image	F4/80 antibody [CI:A3-1] for IHC-Fr (Mouse) Copyright image used under license

review by Sarah Barnes submitted on 16 October 2006
Application:	Immunohistochemistry (Frozen sections)
F4/80 antibody [CI:A3-1] (ab6640)
Sample	Mouse Tissue sections (Liver)
Specification	Liver
Fixative:	Paraformaldehyde
Blocking step:	Serum as blocking agent for  1 hour  · Concentration: 5%
Primary Antibody
Dilution	1/100
Incubation time:	2 hours
Secondary Antibody
Name:	Non-Abcam Antibody was used: Goat anti-rat AlexiFluor 488 IgG Conjugation: Alexa Fluor® 488
Dilution:	1/100
Additional Data
Rating	Good
Image	F4/80 antibody [CI:A3-1] for IHC-Fr (Mouse) Copyright image used under license

review by Eva Lund submitted on 20 February 2003
Application:	Immunohistochemistry (Frozen sections)
F4/80 antibody [CI:A3-1] (ab6640)
Additional Data
Notes	We have used the antibody for immunostaining of frozen sections of mouse tumors and wounds.The best results was obtained with a 1:1000 dilution in TBS-BSA.Secondary ab: rabbit anti-rat 1:600(E468, DAKO), followed by HRP-streptavidin, 1:500(DAKO) and Nova red. It worked very well. We also stained tissue prefixed with Stefani's fixative with good results.
Dilution notes	Primary ab: 1:1000 dilution in TBS-BSA. Secondary ab: rabbit anti-rat 1:600(E468, DAKO) Tert: ab: HRP-streptavidin, 1:500(DAKO)