68kDa Neurofilament antibody - Neuronal Marker (ab9035)

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review by Luis Craveiro submitted on 30 June 2006
Application:	Immunocytochemistry/ Immunofluorescence
68kDa Neurofilament antibody - Neuronal Marker (ab9035)
Sample	Rat Cell (Hippocampal Organotypic Slice Cultures)
Specification	Hippocampal Organotypic Slice Cultures
Fixative:	Paraformaldehyde
Blocking step:	Serum as blocking agent for  24 hours  · Concentration: 1.5%
Primary Antibody
Dilution	1/300
Incubation time:	120 hours
Secondary Antibody
Name:	Non-Abcam Antibody was used: Indocarbocyanine, Cy3 Conjugation: Cy3 ®
Dilution:	1/1000
Additional Data
Rating	Good
Additional notes	This image was taken with a 20x objective and is from the stratum oriens of CA1 region of the hippocampus. Occasionally the cell soma and proximal dendrites are also stained (as seen in one cell on the picture) but this is a rare event for CA1 pyramidal cells. Axons projecting from these cells are very nicelly stained and are very weel seen in this area since it is thinner (it is the edge of the culture). The more "diffuse" stainning seen in the stratum pyramidalis (upper part of the image) is due to a greater number of axons present and a greater thickness of the culture. This is not a confocal reconstruction.
Image	68kDa Neurofilament antibody - Neuronal Marker for ICC/IF (Rat) Copyright image used under license

review by Keith Page submitted on 17 October 2003
Application:	Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
68kDa Neurofilament antibody - Neuronal Marker (ab9035)
Additional Data
Notes	Strong and specific immunohostochemical signals when used following citrate-microwave mediated antigen retrieval, in formalin fixed / paraffin embedded CNS and non-CNS human tissues.
Dilution notes	ab9035 in the range 1:500-1:2000 is effective 1:200 universal biotinylated IgG ABC - DAB