Anti-Indoleamine 2, 3-dioxygenase antibody (ab55305)

Overview

  • Product name
    Anti-Indoleamine 2, 3-dioxygenase antibody
    See all Indoleamine 2, 3-dioxygenase primary antibodies
  • Description
    Mouse monoclonal to Indoleamine 2, 3-dioxygenase
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-404 of Human Indoleamine 2, 3-dioxygenase

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab211017 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab55305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22415306
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
  • Pathway
    Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
  • Sequence similarities
    Belongs to the indoleamine 2,3-dioxygenase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-dioxygenase antibody
    • I23O1_HUMAN antibody
    • IDO 1 antibody
    • IDO antibody
    • IDO-1 antibody
    • IDO1 antibody
    • INDO antibody
    • indolamine 2,3 dioxygenase antibody
    • Indole 2 3 dioxygenase antibody
    • indoleamine 2 3 dioxygenase 1 antibody
    • indoleamine 2 3 dioxygenase antibody
    • Indoleamine 2,3-dioxygenase 1 antibody
    • Indoleamine pyrrole 2 3 dioxygenase antibody
    • Indoleamine-pyrrole 2 antibody
    see all

Images

  • Indoleamine 2 3-dioxygenase antibody (ab55305) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab55305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55305, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Indoleamine 2 3-dioxygenase antibody (ab55305) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.

References

This product has been referenced in:
  • Kaewkangsadan V  et al. Tumour-draining axillary lymph nodes in patients with large and locally advanced breast cancers undergoing neoadjuvant chemotherapy (NAC): the crucial contribution of immune cells (effector, regulatory) and cytokines (Th1, Th2) to immune-mediated tumour cell death induced by NAC. BMC Cancer 18:123 (2018). IHC-P ; Human . Read more (PubMed: 29390966) »
  • Rihn SJ  et al. The Envelope Gene of Transmitted HIV-1 Resists a Late Interferon Gamma-Induced Block. J Virol 91:N/A (2017). Read more (PubMed: 28100611) »

See all 13 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate Cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Permeabilization
No
Specification
Prostate Cancer
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 12 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Permeabilization
No
Specification
Skin melanoma
Blocking step
Dako Endogenous Peroxidase Block as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Thank you for contacting us.

I have found a research paper about Indoleamine 2,3-Dioxygenase tissue distribution and cellular localization that may be useful to determine the cellular localization.

Indoleamine 2,3-Dioxygenase Tiss...

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Thank you for your email.

I had sent a reply to you 5 minutes before I received your second email. Our emails must have crossed each other on the way.

For ab134197, please see my previous email.

For ab106134, this antibody...

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I am very pleased to hear you would like to accept our offer and test ab55305 and ab106134 in monkey. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for each antibo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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