Overview

  • Product nameAnti-Influenza A Virus antibody
    See all Influenza A Virus primary antibodies
  • Description
    Goat polyclonal to Influenza A Virus
  • SpecificitySpecific to H1N1 by IHA. Detects Influenza A strain PR/8/34 by IFA. Does not react Influenza B, RSV, Parainfluenza 1-3 or Adenovirus. Does not react with HEp-2 cells.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Conjugationmore details
  • Species reactivity
    Reacts with Influenza A Virus. May react with chicken cellular proteins. Not yet tested in other species.
  • Immunogen

    Influenza A virus. Strain: USSR (H1N1).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notes>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/300.

This product may be used in place of neat antiserum in almost any appropriate antibody-based technique.

Conjugation Use at an assay dependent concentration.

Target

  • RelevanceInfluenza A and B are the two types of influenza viruses that cause epidemic human disease. Influenza type C infections cause a mild respiratory illness and are not thought to cause epidemics. Influenza A viruses are further categorized into subtypes on the basis of two surface antigens: hemagglutinin (H) and neuraminidase (N). Strains are also described by geographic origin, strain number and year of isolation.
  • Cellular localizationNuclear

Anti-Influenza A Virus antibody images

  • ab20841 staining Influenza A Virus in reconstructed 1918 H1N1 influenza infected Mouse lung tissue sections by Immunohistochemistry. Tissue was fixed with 10% neutral-buffered formalin and embedded in paraffin. Slides were deparaffinized, rehydrated in water, steam antigen retrieved in pH 6 citrate buffer, and blocked with 3% H2O2. Samples were incubated with primary antibody (1/500) for 1 hour at room temperature. Top - EUK-207 treated mouse, 8 days postinfection, Bottom uninfected mouse control. Brown staining indicates positive staining in cell's cytoplasm and/or nucleus. Cellular debris is also frequently positive.

References for Anti-Influenza A Virus antibody (ab20841)

This product has been referenced in:
  • Khaperskyy DA  et al. Influenza a virus host shutoff disables antiviral stress-induced translation arrest. PLoS Pathog 10:e1004217 (2014). ICC/IF . Read more (PubMed: 25010204) »
  • Qi L  et al. Contemporary avian influenza A virus subtype H1, H6, H7, H10, and H15 hemagglutinin genes encode a mammalian virulence factor similar to the 1918 pandemic virus H1 hemagglutinin. MBio 5:e02116 (2014). IHC ; Mouse . Read more (PubMed: 25406382) »

See all 10 Publications for this product

Product Wall

Thank you for contacting Abcam regarding ab20841.


I have confirmed that this antibody is against all proteinsin H1N1 influenza A virus and the alternative name was added in error. The antibody does not specifically target the M1 protein...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"