Overview

  • Product name
    Anti-Influenza A Virus antibody
    See all Influenza A Virus primary antibodies
  • Description
    Goat polyclonal to Influenza A Virus
  • Specificity
    Specific to H1N1 by IHA. Detects Influenza A strain PR/8/34 by IFA. Does not react Influenza B, RSV, Parainfluenza 1-3 or Adenovirus. Does not react with HEp-2 cells.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Conjugationmore details
  • Species reactivity
    Reacts with Influenza A Virus. May react with chicken cellular proteins. Not yet tested in other species.
  • Immunogen

    Influenza A virus. Strain: USSR (H1N1).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    >95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/300.

This product may be used in place of neat antiserum in almost any appropriate antibody-based technique.

Conjugation Use at an assay dependent concentration.

Target

  • Relevance
    Influenza A and B are the two types of influenza viruses that cause epidemic human disease. Influenza type C infections cause a mild respiratory illness and are not thought to cause epidemics. Influenza A viruses are further categorized into subtypes on the basis of two surface antigens: hemagglutinin (H) and neuraminidase (N). Strains are also described by geographic origin, strain number and year of isolation.
  • Cellular localization
    Nuclear

Anti-Influenza A Virus antibody images

  • ab20841 staining Influenza A Virus in reconstructed 1918 H1N1 influenza infected Mouse lung tissue sections by Immunohistochemistry. Tissue was fixed with 10% neutral-buffered formalin and embedded in paraffin. Slides were deparaffinized, rehydrated in water, steam antigen retrieved in pH 6 citrate buffer, and blocked with 3% H2O2. Samples were incubated with primary antibody (1/500) for 1 hour at room temperature. Top - EUK-207 treated mouse, 8 days postinfection, Bottom uninfected mouse control. Brown staining indicates positive staining in cell's cytoplasm and/or nucleus. Cellular debris is also frequently positive.

References for Anti-Influenza A Virus antibody (ab20841)

This product has been referenced in:
  • Khaperskyy DA  et al. Selective Degradation of Host RNA Polymerase II Transcripts by Influenza A Virus PA-X Host Shutoff Protein. PLoS Pathog 12:e1005427 (2016). WB, IF . Read more (PubMed: 26849127) »
  • Peteranderl C  et al. Macrophage-epithelial paracrine crosstalk inhibits lung edema clearance during influenza infection. J Clin Invest 126:1566-80 (2016). Read more (PubMed: 26999599) »

See all 14 Publications for this product

Product Wall

Thank you for contacting Abcam regarding ab20841.


I have confirmed that this antibody is against all proteinsin H1N1 influenza A virus and the alternative name was added in error. The antibody does not specifically target the M1 protein...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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