Anti-Influenza A Virus Hemagglutinin antibody [1.B.408] (ab119966)

Overview

• Product name
  Anti-Influenza A Virus Hemagglutinin antibody [1.B.408]
  See all Influenza A Virus Hemagglutinin primary antibodies
• Description
  Mouse monoclonal [1.B.408] to Influenza A Virus Hemagglutinin
• Specificity
  ab119966 Reacts with hemagglutinin of H1 serotype.
• Tested applications
  Suitable for: ELISA, ICC/IF, Inhibition Assay, ICCmore details
• Species reactivity
  Reacts with: Influenza A
  
• Immunogen

  Purified influenza virus type A strain H1N1.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• Storage buffer
  pH: 7.40
  Preservative: 0.1% Sodium azide
  Constituent: 99% PBS
• Concentration information loading...
• Purity
  Protein G purified
• Purification notes
  Purified by Protein G affinity chromatography (? 95% SDS-PAGE).
• Clonality
  Monoclonal
• Clone number
  1.B.408
• Isotype
  IgG1
• Research areas
  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Microbiology
  • Organism
  • Virus
  • RNA Virus
  • single stranded RNA negative strand virus
  • Influenza

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Target

• Relevance
  Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong. There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species jumping ability. Influenza A Virus Hemagglutinin antibodies recognize the influenza hemagglutinin epitope, which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of this antibody allows for unambiguous identification and quantitative analysis of the tagged protein.
• Cellular localization
  Apical cell membrane; Single-pass type I membrane protein. Note=Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.
• Database links
  • Entrez Gene: 956529 Influenza A
  • SwissProt: P03437 Influenza A
  • SwissProt: P03452 Influenza A
  • SwissProt: Q9WFX3 Influenza A
• Alternative names
  • HA antibody
  • HA1 antibody
  • HA2 antibody
  • Hemagglutinin antibody

Protocols

• Immunocytochemistry & immunofluorescence protocols