The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 1 µg/ml. ab91531 will detect 2 ng of free peptide at 1 µg/ml.
WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 63 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceInfluenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes.
In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells.
Hemagglutinin HA antibody
Anti-Influenza A Virus Hemagglutinin H1 antibody images
Western blot - Influenza A Virus Hemagglutinin H1 antibody (ab91531)
All lanes : Anti-Influenza A Virus Hemagglutinin H1 antibody (ab91531) at 2 µg/ml
Lane 1 : Recombinant Influenza A Virus Hemagglutinin H1 Lane 2 : Recombinant Swine A Virus Hemagglutinin H1
Predicted band size : 63 kDa
References for Anti-Influenza A Virus Hemagglutinin H1 antibody (ab91531)
This product has been referenced in:
Price JV et al. Characterization of influenza vaccine immunogenicity using influenza antigen microarrays. PLoS One8:e64555 (2013).
Read more (PubMed: 23734205) »