Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189)


  • Product name
    Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M]
    See all Influenza A Virus Hemagglutinin H1 primary antibodies
  • Description
    Mouse monoclonal [B219M] to Influenza A Virus Hemagglutinin H1
  • Host species
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Influenza A Virus.
  • Immunogen

    Purified Influenza A/NewCaledonia/20/99 virus (strain H1N1)

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab91531 as a replacement.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >90% pure (SDS-PAGE).
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab66189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 5 µg/ml.


  • Relevance
    Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells.
  • Alternative names
    • HA antibody
    • Hemagglutinin antibody
    • Hemagglutinin HA antibody


  • Anti-Influenza A Virus Hemagglutinin H1 antibody [B219M] (ab66189) at 5 µg/ml + Influenza A/Taiwan/1/86 at 0.5 µg

    HRP-conjugated anti-mouse IgG

    Performed under reducing conditions.

    Protocol notes:

    • SDS-PAGE in reducing conditions, 10 % acrylamide
    • After SDS-PAGE transfer to nitrocellulose membrane
    • Blocking in PBS, containing 0.5% Tween 20, 10% dry milk (w/v) – incubation 10 minutes at room temperature.
    • Primary antibody: 5 µg/ml diluted in PBS containing 0.5% Tween 20 – incubation 1 hour at room temperature.
    • Secondary antibody: HRP-conjugated anti-mouse IgG diluted in PBS, containing 0.5% Tween 20 – incubation 1hour at room temperature.
    • Diaminobenzidine 50 µg/ml – 20 min


This product has been referenced in:
  • Ahmed SR  et al. In situ self-assembly of gold nanoparticles on hydrophilic and hydrophobic substrates for influenza virus-sensing platform. Sci Rep 7:44495 (2017). Read more (PubMed: 28290527) »
  • Price JV  et al. Characterization of influenza vaccine immunogenicity using influenza antigen microarrays. PLoS One 8:e64555 (2013). Read more (PubMed: 23734205) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam. The free of charge replacement of ab66189 with ab119981 has been placed on order xxxx as you have requested. I hope that this information is helpful. Please let me know if you have any questions or there are ot...

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Thanks for the additional information.

It does appear as if these two antibodies are not working as expected in ELISA. I would be happy to replace them for you with any other primary antibodies.

I will just need the order reference ...

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Thanks so much for the additional information.

It looks like ab66189 and ab61986 are being use as detection antibodies which are in turn detected by anti-mouse secondary antibodies? Is this the case?

If so I was wondering how much o...

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Thank you for your enquiry and for completing the questionnaire.

I just have a few follow up questions:

1. Please describe the sample loaded onto well?

2. Did other antibodies work in ELISA assay with this same sample? Read More

I would still need the accession number for your strain, but I just wanted to let you know of additional information about 2 of our products.

Concerning ab61986, this antibody recognizes all Hemagglutinin from H1N1 strains. The immunoge...

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