Anti-Influenza A Virus M2 Protein antibody (ab56086)

Overview

  • Product nameAnti-Influenza A Virus M2 Protein antibody
    See all Influenza A Virus M2 Protein primary antibodies
  • Description
    Rabbit polyclonal to Influenza A Virus M2 Protein
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Influenza A
  • Immunogen

    A synthetic peptide corresponding to N terminal residues of Influenza A Virus M2 Protein

Properties

Applications

Our Abpromise guarantee covers the use of ab56086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
    • Cellular localizationVirion membrane. Apical cell membrane; Single-pass type III membrane protein.
    • Database links
      • Alternative names
        • Influenza A virus matrix protein M2 antibody
        • M antibody
        • M2 antibody
        • Matrix protein 2 antibody
        • Membrane ion channel M2 antibody
        • Membrane protein M2 antibody
        • Proton channel protein M2 antibody
        see all

      References for Anti-Influenza A Virus M2 Protein antibody (ab56086)

      This product has been referenced in:
      • Ebrahimi SM  et al. Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2. Mol Biol Rep 37:2909-14 (2010). WB, ICC/IF ; Influenza A . Read more (PubMed: 19809890) »
      • Ebrahimi SM & Tebianian M Heterologous expression, purification and characterization of the influenza A virus M2e gene fused to Mycobacterium tuberculosis HSP70(359-610) in prokaryotic system as a fusion protein. Mol Biol Rep 37:2877-83 (2010). ICC/IF . Read more (PubMed: 19813102) »

      See all 3 Publications for this product

      Product Wall

      Abcam has not validated the combination of species/application used in this Abreview.
      Application ELISA
      Sample A/Sw/SK/2001 (H1N1) influenza Purified protein (Purified inactivated human H3N2 influenza virus)
      Specification Purified inactivated human H3N2 influenza virus
      Type Indirect
      Username

      Abcam user community

      Verified customer

      Submitted Nov 02 2016

      Thank you for your enquiry.

      The immunogens used to produce ab94925 and ab56086 consist of a small 15-20 amino acid peptide from Influenza A virus M2 protein. These peptides are not His tagged.

      I hope this is helpful. Please contact ...

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      Thank you very much for your inquiry. I have contacted the laboratory for information on the ab5416. However the laboratory has not tested this antibody neither on your strain. I did a sequence alignement of the immunogen and your sequence. Please see ...

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      Thank you for contacting us. The species is not applicable for this product so the discount code can’t be given. However please inform customer for submitting an Abreview after using the product. I hope this information is helpful to you. Pleas...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"