The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
Cellular localizationVirion membrane. Apical cell membrane; Single-pass type III membrane protein.
References for Anti-Influenza A Virus M2 Protein antibody (ab56086)
This product has been referenced in:
Kawaguchi A et al. Influenza Virus Induces Cholesterol-Enriched Endocytic Recycling Compartments for Budozone Formation via Cell Cycle-Independent Centrosome Maturation. PLoS Pathog11:e1005284 (2015).
Read more (PubMed: 26575487) »
Ebrahimi SM et al. Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2. Mol Biol Rep37:2909-14 (2010).
Read more (PubMed: 19809890) »