The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 80 kDa.
This antibody has only been tested against the recombinant protein, and not the endogenous form.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1.
"NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
Cellular localizationHost cell nucleus
Common flu NP antibody
Influenza A virus NP antibody
Nucleocapsid protein antibody
Protein N antibody
Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
Anti-Influenza A Virus Nucleoprotein antibody  images
Western blot - Influenza A Virus Nucleoprotein antibody  (ab81126)
Anti-Influenza A Virus Nucleoprotein antibody  (ab81126) at 1/1000 dilution + recombinant
Influenza A virus NP