Anti-Influenza A Virus Nucleoprotein antibody [9G8] (ab43821)

Overview

  • Product nameAnti-Influenza A Virus Nucleoprotein antibody [9G8]
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [9G8] to Influenza A Virus Nucleoprotein
  • SpecificityRecognizes Nucleoprotein of Influenza A virus group specific antigen (H0N1, H1N1, H2N2 and H3N2).
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Influenza A. No cross-reactivity with influenza B or with other respiratory viruses.
  • Immunogen

    Recombinant full length protein corresponding to Influenza A Virus Nucleoprotein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number9G8
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.
WB 1/200 - 1/1000.
ICC/IF 1/100 - 1/500.

On infected cells 

Target

  • RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localizationHost cell nucleus
  • Alternative names
    • Common flu NP antibody
    • Influenza A virus NP antibody
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody
    • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
    see all

References for Anti-Influenza A Virus Nucleoprotein antibody [9G8] (ab43821)

This product has been referenced in:
  • Dimmock NJ  et al. Comparison of the protection of ferrets against pandemic 2009 influenza A virus (H1N1) by 244 DI influenza virus and oseltamivir. Antiviral Res 96:376-85 (2012). Read more (PubMed: 23041142) »

See 1 Publication for this product

Product Wall

Thank you for your response to my further questions. It is very much appreciated.

When experiments are carried out using transfected cells, it is very important to provide information about the construct and any positive controls used to make...

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Thank you for contacting us.

I am sorry if ab128193 did not work as you expected.

H7N3 subtype of avian Influenza A virus has not been tested with this antibody. I would recommend you to test IF with the virus-infected cells or ELIS...

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ab43821 has been produced using a recombinant inlfuenza A protein produced in MDCK (Madin-Darby Canine Kidney) cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"