Anti-Influenza A Virus Nucleoprotein antibody [B247M] (ab66191)


  • Product nameAnti-Influenza A Virus Nucleoprotein antibody [B247M]
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [B247M] to Influenza A Virus Nucleoprotein
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with Influenza A Virus. Does not react with Influenza B Virus.
  • Immunogen

    Purified Influenza virus type A (H1N1)

  • Positive control
    • purified Influenza A antigens (H1N1 and H3N2 strains)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure (SDS-PAGE).
  • ClonalityMonoclonal
  • Clone numberB247M
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab66191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [B248M] to Influenza A Virus Nucleoprotein (ab110661).
WB Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent dilution.


  • RelevanceThe nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localizationHost cell nucleus
  • Alternative names
    • Common flu NP antibody
    • Influenza A virus NP antibody
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody
    • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
    see all

References for Anti-Influenza A Virus Nucleoprotein antibody [B247M] (ab66191)

ab66191 has not yet been referenced specifically in any publications.

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Thank you for your response to my further questions. It is very much appreciated.

When experiments are carried out using transfected cells, it is very important to provide information about the construct and any positive controls used to make...

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Thank you for contacting us. The antibodies have different clone number, concentration is different, purity is different, storage buffer percentage is different, and these are tested in different applications. Although these products are agains...

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ab66191 has been produced using purified Influenza A virus - the immunogen was not a recombinant protein.

Unfortunately we are not aware of any publications for ab66191. However, ab66191 gave a single band against purified Influenza A antigens (H1N1 and H3N2 strains) on the blot (SDS-PAGE, reducing conditions, 1ug antigen/lane). The antibody was tested...

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