Anti-Influenza A Virus Nucleoprotein antibody [B247M] (ab66191)

Overview

  • Product name
    Anti-Influenza A Virus Nucleoprotein antibody [B247M]
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [B247M] to Influenza A Virus Nucleoprotein
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with Influenza A Virus. Does not react with Influenza B Virus.
  • Immunogen

    Purified Influenza virus type A (H1N1)

  • Positive control
    • purified Influenza A antigens (H1N1 and H3N2 strains)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >90% pure (SDS-PAGE).
  • Clonality
    Monoclonal
  • Clone number
    B247M
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
  • Cellular localization
    Host cell nucleus
  • Alternative names
    • Common flu NP antibody
    • Influenza A virus NP antibody
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody
    • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
    see all

References

This product has been referenced in:
  • Wu CY  et al. A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus. PLoS One 7:e42363 (2012). Read more (PubMed: 22879951) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your response to my further questions. It is very much appreciated.

When experiments are carried out using transfected cells, it is very important to provide information about the construct and any positive controls used to make...

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The performa #46714 has been sent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview....

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Thank you for contacting us. The antibodies have different clone number, concentration is different, purity is different, storage buffer percentage is different, and these are tested in different applications. Although these products are agains...

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ab66191 has been produced using purified Influenza A virus - the immunogen was not a recombinant protein.

Unfortunately we are not aware of any publications for ab66191. However, ab66191 gave a single band against purified Influenza A antigens (H1N1 and H3N2 strains) on the blot (SDS-PAGE, reducing conditions, 1ug antigen/lane). The antibody was tested...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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