Anti-Influenza A Virus Nucleoprotein antibody [F8] (ab8261)


  • Product name
    Anti-Influenza A Virus Nucleoprotein antibody [F8]
    See all Influenza A Virus Nucleoprotein primary antibodies
  • Description
    Mouse monoclonal [F8] to Influenza A Virus Nucleoprotein
  • Host species
  • Specificity
    This antibody reacts with nucleoprotein (NP), which is common for Influenza A(H1N1), A(H3N2) and other Influenza A viruses.
  • Tested applications
    Suitable for: ELISA, ICCmore details
  • Immunogen

    Purified influenza virus type A strain H1N1.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab20343 as a replacement.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is affinity purified.
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab8261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.

    Can also be used for detection and serotyping of influenza virus type A.
    Inhibit viral reproduction after fatty acid acylation.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    ICC protocol:
    1)Cell monolayer was grown on a glass and infected with the virus for 2 days at 37 oC.
    2)Glass was dried and the cells were fixed with cold acetone at 4 oC for 10 min.
    3)Ab8261 was introduced in concentrations from 1 to 100 µg/ml in PBS and incubated for 30 min in RT.
    4)Glass was washed with PBS several times and incubated with goat anti-mouse IgG labeled with FITC.
  • Target

    • Relevance
      The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
    • Cellular localization
      Host cell nucleus
    • Alternative names
      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all


    This product has been referenced in:
    • Yan Y  et al. Human nasal epithelial cells derived from multiple subjects exhibit differential responses to H3N2 influenza virus infection in vitro. J Allergy Clin Immunol 138:276-281.e15 (2016). Read more (PubMed: 26806046) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for getting back to me. The concentration of ab8261 needs to be determined by the end user. I would recommend that you perform a series of dilutions and adjust your future experiments thereof. We have some comprehensive ELISA protocols...

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    Thank you for your email. Looking at the datasheet for ab5416, it states that the antibody has been tested for application in Western blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. To our knowledge, it has not yet been test...

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