Anti-Influenza B Virus antibody [2B2] (ab54142)

Overview

  • Product nameAnti-Influenza B Virus antibody [2B2]
    See all Influenza B Virus primary antibodies
  • Description
    Mouse monoclonal [2B2] to Influenza B Virus
  • SpecificityThis antibody is specific for nuclear protein hence gives a 'nuclear' staining in IF.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with Influenza B virus.
  • Immunogen

    Native

Properties

Applications

Our Abpromise guarantee covers the use of ab54142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/300 - 1/15000.
ICC/IF 1/300 - 1/15000.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceInfluenza A and B are the two types of influenza viruses that cause epidemic human disease. Influenza B viruses currently circulating are divided into two antigenically and genetically distinct groups: the Victoria and Yamagata lineages.
  • Cellular localizationNuclear
  • Alternative names
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody

References for Anti-Influenza B Virus antibody [2B2] (ab54142)

ab54142 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (A549 cell line)
Specification A549 cell line
Preparation Cell harvesting/tissue preparation method: Trypsinised and spun down.
Sample buffer: Trypsin + EDTA
Fixation BD FIx & Perm. Kit
Permeabilization Yes - BD FIx & Perm. Kit
Gating Strategy Single cell suspension, gated on live cells (Hoechst positive).
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Submitted Dec 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"