Recombinant Anti-ING1 antibody [EPR6788] (ab124893)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6788] to ING1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ING1 antibody [EPR6788]
See all ING1 primary antibodies -
Description
Rabbit monoclonal [EPR6788] to ING1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 lysate, HeLa lysate, MCF-7 lysate, Jurkat lysate, C6 lysate, RAW264.7 lysate, PC-12 lysate and NIH/3T3 lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6788 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
Target
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Function
Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene. -
Tissue specificity
Isoform 2 was expressed in all normal tissues and cells examined, as well as in all breast cancer and melanoma cell lines examined. Isoform 3 was expressed in testis, liver, and kidney, weakly expressed in colon and brain and not expressed in breast and cultured melanocytes. Isoform 4 was highly expressed in testis and weakly expressed in brain, but not expressed in breast, colon, kidney, melanocytes, breast cancer or melanoma cell lines. -
Involvement in disease
Defects in ING1 are a cause of head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck. -
Sequence similarities
Belongs to the ING family.
Contains 1 PHD-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3621 Human
- Entrez Gene: 26356 Mouse
- Entrez Gene: 306626 Rat
- Omim: 601566 Human
- SwissProt: Q9UK53 Human
- SwissProt: Q9QXV3 Mouse
- Unigene: 46700 Human
- Unigene: 508725 Human
see all -
Alternative names
- 2610028J21Rik antibody
- AA407184 antibody
- AI875420 antibody
see all
Images
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All lanes : Anti-ING1 antibody [EPR6788] (ab124893) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : MCF-7 lysate
Lane 4 : Jurkat lysate
Lane 5 : C6 lysate
Lane 6 : RAW 264.7 lysate
Lane 7 : PC-12 lysate
Lane 8 : NIH/3T3 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 47 kDa -
Intracellular flow cytometric analysis of ab124893, at 1/10 dilution, staining ING1 in permeabilised NIH/3T3cells (red). Isotype control (Green).
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124893 has been referenced in 3 publications.
- Garcia-Campos MA et al. Deciphering the "m6A Code" via Antibody-Independent Quantitative Profiling. Cell 178:731-747.e16 (2019). PubMed: 31257032
- Yan H & Wu A FOXO1 is crucial in glioblastoma cell tumorigenesis and regulates the expression of SIRT1 to suppress senescence in the brain. Mol Med Rep 17:2535-2542 (2018). PubMed: 29207098
- Kim SW et al. Latent progenitor cells as potential regulators for tympanic membrane regeneration. Sci Rep 5:11542 (2015). IHC-Fr ; Rat . PubMed: 26100219