General notesPrincipal Names - ING1L; inhibitor of growth family, member 1-like; p33ING2; inhibitor of growth 1-like
Official Gene Symbol - ING1L
GenBank Accession Number – NP_001555
LocusLink ID - 3622 (human)
Gene Ontology terms - chromatin binding; signal transduction; chromosome
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 32.8 kDa).Can be blocked with Human ING2 peptide (ab22925).
Use at an assay dependent dilution.
FunctionSeems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
Tissue specificityWidely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
Sequence similaritiesBelongs to the ING family. Contains 1 PHD-type zinc finger.
DomainThe PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
Cellular localizationNucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.