Recombinant
RabMAb

Anti-ING2 antibody [EPR4597] (ab109504)

Overview

  • Product name
    Anti-ING2 antibody [EPR4597]
    See all ING2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4597] to ING2
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • WB: T47-D, HepG2 or HT-1080 cell lysates IHC-P: Human kidney tissue This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IP 1/10 - 1/100.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
    • Tissue specificity
      Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
    • Sequence similarities
      Belongs to the ING family.
      Contains 1 PHD-type zinc finger.
    • Domain
      The PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
    • Cellular localization
      Nucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ING 1 like tumor suppressor protein antibody
      • ING 1L antibody
      • ING 2 antibody
      • ING1 like tumor suppressor protein antibody
      • ING1L antibody
      • ING1Lp antibody
      • ING2 antibody
      • ING2_HUMAN antibody
      • Inhibitor of growth 1 like antibody
      • Inhibitor of growth 1 like protein antibody
      • Inhibitor of growth 1-like protein antibody
      • Inhibitor of growth family member 1 like antibody
      • Inhibitor of growth family member 2 antibody
      • Inhibitor of growth protein 2 antibody
      • p32 antibody
      • p33 ING2 antibody
      • p33ING2 antibody
      see all

    Images

    • ICC/IF image of ab109504 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109504 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-ING2 antibody [EPR4597] (ab109504) at 1/10000 dilution

      Lane 1 : T47-D cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 33 kDa
    • Immunohistochemical analysis of ING2 expression in formalin-fixed, paraffin-embedded Human kidney tissue using ab109504 at 1/1000 dilution.

    References

    ab109504 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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