1/1000 - 1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt.
FunctionSeems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
Tissue specificityWidely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
Sequence similaritiesBelongs to the ING family. Contains 1 PHD-type zinc finger.
DomainThe PHD-type zinc finger domain binds to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).
Cellular localizationNucleus. Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
ICC/IF image of ab109504 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109504 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - ING2 antibody [EPR4597] (ab109504)
All lanes : Anti-ING2 antibody [EPR4597] (ab109504) at 1/10000 dilution
Lane 1 : T47-D cell lysate Lane 2 : HepG2 cell lysate Lane 3 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary Standard HRP labelled goat anti-rabbit at 1/2000 dilution