Overview

  • Product name
  • Description
    Goat polyclonal to ING3
  • Specificity
    ab115470 is specific for isoform 1 of ING3.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    ADSRSGRKSKN

    conjugated to KLH, corresponding to amino acids 294-304 of Human ING3.

  • Positive control
    • Human brain (cortex) tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab115470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 47 kDa.
ELISA 1/10000 - 1/40000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    Function: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. Tissue specificity: Expressed in brain, heart, kidney, liver, lung, ovaries, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Disease: Squamous cell carcinoma of the head and neck Similarity: Belongs to the ING family. Contains 1 PHD-type zinc finger. Domain: The PHD-type zinc finger mediates the binding to H3K4me3.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • 1300013A07Rik antibody
    • Eaf 4 antibody
    • Eaf4 antibody
    • FLJ20089 antibody
    • ING 2 antibody
    • ING 3 antibody
    • ING2 antibody
    • ING3 antibody
    • ING3_HUMAN antibody
    • Inhibitor of growth family member 3 antibody
    • Inhibitor of growth protein 3 antibody
    • MEAF4 antibody
    • p47 ING 3 antibody
    • p47 ING3 antibody
    • p47 regulator protein antibody
    • p47ING3 antibody
    • p47ING3 protein antibody
    see all

Anti-ING3 antibody images

  • ab115470 at 2.5ug/ml staining ING3 in Formalin-fixed, Paraffin-embedded Human brain (cortex) tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-ING3 antibody (ab115470)

ab115470 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (embryo 10.5 dpc)
Specification
embryo 10.5 dpc
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Dieter Fink

Verified customer

Submitted Oct 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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