Overview

  • Product nameAnti-ING3 antibody
    See all ING3 primary antibodies
  • Description
    Goat polyclonal to ING3
  • Specificityab115470 is specific for isoform 1 of ING3.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    ADSRSGRKSKN

    conjugated to KLH, corresponding to amino acids 294-304 of Human ING3.

  • Positive control
    • Human brain (cortex) tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab115470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 47 kDa.
ELISA 1/10000 - 1/40000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceING3 belongs to a family of proteins containing the plant homeodomain (PHD) finger, which includes transcription factors and proteins that regulate chromatin structure. ING3 is a component of the NuA4 histone acetyltransferase (HAT) complex and can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of ING3 has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of the ING3 gene were detected in head and neck cancers.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 1300013A07Rik antibody
    • Eaf 4 antibody
    • Eaf4 antibody
    • FLJ20089 antibody
    • ING 2 antibody
    • ING 3 antibody
    • ING2 antibody
    • Inhibitor of growth family member 3 antibody
    • Inhibitor of growth protein 3 antibody
    • MEAF4 antibody
    • p47 ING 3 antibody
    • p47 ING3 antibody
    • p47 regulator protein antibody
    • p47ING3 antibody
    • p47ING3 protein antibody
    see all

Anti-ING3 antibody images

  • ab115470 at 2.5ug/ml staining ING3 in Formalin-fixed, Paraffin-embedded Human brain (cortex) tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-ING3 antibody (ab115470)

ab115470 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (embryo 10.5 dpc)
Specification embryo 10.5 dpc
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Dieter Fink

Verified customer

Submitted Oct 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"