Overview

  • Product nameAnti-ING4 antibody
    See all ING4 primary antibodies
  • Description
    Goat polyclonal to ING4
  • SpecificityTested on a U2OS cell line overexpressing human ING4.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    CLVRTSPEYGMPS

    , corresponding to amino acids 163 - 174 of Human ING4.

  • General notes


    ING4 is moderately similar to ING1 and may act in chromatin-mediated transcriptional regulation. ING4 contains a PHD-finger.

Properties

Applications

Our Abpromise guarantee covers the use of ab3714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

Target

  • FunctionComponent of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1).
  • Sequence similaritiesBelongs to the ING family.
    Contains 1 PHD-type zinc finger.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain my036 protein antibody
    • Candidate tumor suppressor p33 ING 1 homolog antibody
    • Candidate tumor suppressor p33 ING1 homolog antibody
    • D6Wsu147e antibody
    • D6Xrf92 antibody
    • ING 1 like protein antibody
    • ING 4 antibody
    • ING1 like protein antibody
    • ING4 antibody
    • ING4_HUMAN antibody
    • Inhibitor of growth family member 4 antibody
    • Inhibitor of growth family member 4 long isoform antibody
    • Inhibitor of growth protein 4 antibody
    • MGC12557 antibody
    • my036 antibody
    • p29 ING 4 antibody
    • p29 ING4 antibody
    • p29ING4 antibody
    see all

Anti-ING4 antibody images



  • Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


  • Predicted band size : 29 kDa


    Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates. This antibody was used at 1.0 ug/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells.

References for Anti-ING4 antibody (ab3714)

This product has been referenced in:
  • Culurgioni S  et al. Crystal structure of inhibitor of growth 4 (ING4) dimerization domain reveals functional organization of ING family of chromatin-binding proteins. J Biol Chem 287:10876-84 (2012). WB . Read more (PubMed: 22334692) »
  • Conner J & Braidwood L Expression of inhibitor of growth 4 by HSV1716 improves oncolytic potency and enhances efficacy. Cancer Gene Ther 19:499-507 (2012). WB . Read more (PubMed: 22595793) »

See all 5 Publications for this product

Product Wall

Thank you for your enquiry and for submitting your protocol details. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire in association with your blot images and I would appreciate i...

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Thank you for your enquiry. I'm glad to hear that the antibody is giving good preliminary results in IHC. I hope that you will submit an Abreview with your results. Regarding the Western blot, the recommended starting dilution range is between 1/5...

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Thank you for your enquiry. We would like to emphasize the fact that this is a Fast track antibody and it has only been assayed by ELISA against 0.1µg of the immunizing peptide. It has not been tested for Western blot analysis at all. We have r...

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At the bottom of the online datasheet for ab3714 there is a list of compatible secondary antibodies and so yes, ab6742 is an appropriate choice.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"