Anti-ING4 antibody [EP3804] (ab108621)
Key features and details
- Rabbit monoclonal [EP3804] to ING4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-ING4 antibody [EP3804]
See all ING4 primary antibodies -
Description
Rabbit monoclonal [EP3804] to ING4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab197784) -
Positive control
- WB: 293T, Raw264.7, and PC-12 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3804 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human ING4 peptide (ab197784).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human ING4 peptide (ab197784). |
Target
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Function
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). -
Sequence similarities
Belongs to the ING family.
Contains 1 PHD-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51147 Human
- Entrez Gene: 28019 Mouse
- Entrez Gene: 297597 Rat
- Omim: 608524 Human
- SwissProt: Q9UNL4 Human
- SwissProt: Q8C0D7 Mouse
- Unigene: 524210 Human
- Unigene: 262547 Mouse
see all -
Alternative names
- Brain my036 protein antibody
- Candidate tumor suppressor p33 ING 1 homolog antibody
- Candidate tumor suppressor p33 ING1 homolog antibody
see all
Images
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All lanes : Anti-ING4 antibody [EP3804] (ab108621) at 1/1000 dilution (Purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab108621 has been referenced in 4 publications.
- Tang Y et al. ING4 Promotes Stemness Enrichment of Human Renal Cell Carcinoma Cells Through Inhibiting DUSP4 Expression to Activate the p38 MAPK/type I IFN-Stimulated Gene Signaling Pathway. Front Pharmacol 13:845097 (2022). PubMed: 35496267
- Lu L et al. Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1a and snail in HK2 cells. Acta Histochem 121:695-703 (2019). PubMed: 31239073
- Berger PL et al. Miz1, a Novel Target of ING4, Can Drive Prostate Luminal Epithelial Cell Differentiation. Prostate 77:49-59 (2017). PubMed: 27527891
- Pardo M et al. Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells. Sci Rep 7:8157 (2017). PubMed: 28811661