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CGLKKGRGQKEKRGS, corresponding to amino acids 127-140 of Human ING5.
ING5 acts as a tumor suppressor and is a potent growth regulator in normal and in established cells. ING5 may have involvement in chromatin remodeling.
Our Abpromise guarantee covers the use of ab3716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|WB||1/500. Predicted molecular weight: 28 kDa.|
|ICC/IF||Use at an assay dependent dilution. PubMed: 21177815|
This image is courtesy of Jacques Cote
ING5 antibody (ab3716) was used at 1:500 and detected using chemiluminescence reagent. Exposure 5 minutes.
Lane 1: Mock purification
Lane 2: Purified TAP tagged ING4 protein
Lane 3: Purified TAP tagged ING5 from Hela nuclear exracts.
This WB shows the ING5 Ab working on purified material. ab3716 does not recognize ING4 on the same membrane which shows the antibody is specific as ING4 and ING5 are very similar proteins.
ING5 is detected at 44kD due to the TAP tag (predicted size is 28kD).