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Ni-NTA purified truncated recombinant Inhibin alpha expressed in E. Coli strain BL21 (DE3)
Our Abpromise guarantee covers the use of ab47720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||1/200 - 1/1000.|
|WB||1/500 - 1/1000. Predicted molecular weight: 40 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/200 - 1/1000.|
ab47720 at 1/1000 dilution staining Inhibin alpha in human Hela cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary antibody. The primary show green staining in image while actin filaments have been labelled red with DY-554 phalloidin. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.
ab47720 has not yet been referenced specifically in any publications.