Recombinant Anti-Inhibin alpha antibody [EPY2782] (ab81322)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPY2782] to Inhibin alpha
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Inhibin alpha antibody [EPY2782]
See all Inhibin alpha primary antibodies -
Description
Rabbit monoclonal [EPY2782] to Inhibin alpha -
Host species
Rabbit -
Specificity
Note: 20/23 kDa bands consist of the mature alpha chain. 50/53 kDa bands are the entire proprotein. -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Inhibin alpha aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P05111 -
Positive control
- Human Adrenal gland, HeLa, Human placenta and LNCaP lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPY2782 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 40 kDa.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 40 kDa. |
Target
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Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
Tissue specificity
Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsProteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3623 Human
- Entrez Gene: 16322 Mouse
- Entrez Gene: 24504 Rat
- Omim: 147380 Human
- SwissProt: P05111 Human
- SwissProt: Q04997 Mouse
- SwissProt: P17490 Rat
- Unigene: 407506 Human
see all -
Alternative names
- A inhibin subunit antibody
- A inhibin subunit precursor antibody
- AW55578 antibody
see all
Images
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All lanes : Anti-Inhibin alpha antibody [EPY2782] (ab81322) at 1/2000 dilution
Lane 1 : Human Adrenal gland lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human placenta lysate
Lane 4 : LNCaP lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40,50 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Note: the 50 kDa band corresponds to the entire proprotein.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab81322 has been referenced in 1 publication.
- Han L et al. Characterization of the Mechanism of Inhibin a-Subunit Gene in Mouse Anterior Pituitary Cells by RNA Interference. PLoS One 8:e74596 (2013). Mouse . PubMed: 24098340