Overview

  • Product name
    Anti-Inhibin alpha antibody [R1]
    See all Inhibin alpha primary antibodies
  • Description
    Mouse monoclonal [R1] to Inhibin alpha
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human Inhibin alpha aa 233-264. Note that this peptide corresponds to the N-terminus of the mature region of the human inhibin alpha subunit.
    Sequence:

    STPLMSWPWSPSALRLLQRPPEEPAAHANCHR


    Database link: P05111

  • Positive control
    • human ovary, testis, placenta
  • General notes


    Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.08% Sodium Azide
    Constituents: Tissue Culture Supernatant
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.
  • Clonality
    Monoclonal
  • Clone number
    R1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Predicted molecular weight: 32 kDa.
IHC-Fr 1/1 - 1/50.
IHC-P 1/1 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Neat - 1/1/50. Sodium citrate pH 6.0 is recommended for antigen retrieval.

Target

  • Function
    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Tissue specificity
    Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Post-translational
    modifications
    Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A inhibin subunit antibody
    • A inhibin subunit precursor antibody
    • AW55578 antibody
    • IHA antibody
    • inhA antibody
    • INHA_HUMAN antibody
    • Inhibin alpha chain antibody
    • Inhibin alpha chain precursor antibody
    • Inhibin alpha subunit antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human ovary tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue (showing sertoli cells, leydig cells and spermatogonia) labeling inhibin alpha with ab14087.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

References

This product has been referenced in:
  • Chen J  et al. Computed tomography and magnetic resonance imaging features of ovarian fibrothecoma. Oncol Lett 14:1172-1178 (2017). Read more (PubMed: 28693292) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Ovary (abnormal))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween 20, pH 6.0
Permeabilization
No
Specification
Ovary (abnormal)
Blocking step
ab127055 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 21°C
Fixative
Formaldehyde
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Submitted Aug 17 2016

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