Anti-Inhibin beta A antibody (ab56057)

Overview

  • Product nameAnti-Inhibin beta A antibody
    See all Inhibin beta A primary antibodies
  • Description
    Rabbit polyclonal to Inhibin beta A
  • Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Inhibin beta A (N terminal). A synthetic peptide corresponding to N-terminal residues of human INHBA (Inhibin beta A chain precursor)
    Database link: P08476

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab56057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.Can be blocked with Inhibin beta A peptide (ab215854). This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionInhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activin beta-A chain antibody
    • EDF antibody
    • Erythroid differentiation protein antibody
    • INHBA antibody
    • INHBA_HUMAN antibody
    • Inhibin beta A chain antibody
    see all

Anti-Inhibin beta A antibody images

  • ab56057 staining Inhibin in human testicular cancer (seminoma) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked for 20 minutes at 26°C; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An undiluted HRP-conjugated mouse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-Inhibin beta A antibody (ab56057) at 1/1000 dilution + Human COV434 whole cell lysate at 60 µg

    Secondary
    IRDye® 680LT-conjugated goat anti-rabbit IgG polyclonal at 1/25000 dilution

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Francesco Elia Marino

    See Abreview

  • ab56057 staining Inhibin beta A in human COV434 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with ethanol and blocked for 3 hours at 26°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 21°C. A IRDye® 800CW-conjugated goat anti-rabbit IgG (H+L) polyclonal (1/15000) was used as the secondary antibody.

    See Abreview

References for Anti-Inhibin beta A antibody (ab56057)

ab56057 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Odyssey Blocking Buffer as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample Human Cell (COV434)
Specification COV434
Permeabilization Yes - Ethanol
Fixative Formaldehyde
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

Submitted May 28 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step CAS Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Sample Human Tissue sections (Human testis)
Specification Human testis
Permeabilization No
Fixative Bouin's
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

Submitted May 12 2014

Application Western blot
Loading amount 60 µg
Gel Running Conditions Non-reduced Denaturing (12)
Sample Human Cell lysate - whole cell (COV434 Cell Line)
Specification COV434 Cell Line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

Submitted Mar 12 2014

Thank you for your enquiry and your interest in our products. Unit size, price, availability, tested applications and species can be found on the on-line datasheets: http://www.abcam.com/index.html?datasheet=4241 http://www.abcam.com/index.html...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"