The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 47 kDa).
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Follicle stimulating hormone releasing protein antibody
FSH releasing protein antibody
Inhibin beta A chain antibody
Inhibin beta A subunit antibody
Inhibin, beta 1 antibody
Inhibin, beta A (activin A, activin AB alpha polypeptide) antibody
Western blot - Anti-Inhibin beta A antibody (ab93847)
All lanes : Anti-Inhibin beta A antibody (ab93847) at 1 µg/ml
Lane 1 : Human ovary tissue lysate - total protein (ab30222) Lane 2 : Human liver tissue lysate - total protein (ab29889) Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
The band observed at 44 kDa could potentially be a cleaved form of Inhibin beta A due to the presence of a 20 amino acid signal peptide.
Immunocytochemistry/ Immunofluorescence - Anti-Inhibin beta A antibody (ab93847)
ICC/IF image of ab93847 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93847 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol (100%, 5minutes) fixed MCF-7 cells at 5ug/ml.