Anti-Inhibin beta C chain antibody [betaC clone 1] (ab73904)

Overview

  • Product name
    Anti-Inhibin beta C chain antibody [betaC clone 1]
    See all Inhibin beta C chain primary antibodies
  • Description
    Mouse monoclonal [betaC clone 1] to Inhibin beta C chain
  • Specificity
    This antibody has been shown to recognise both monomeric and dimeric Activin (beta C) and does not cross react with BetaAf, BetaB or BetaE.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Inhibin beta C chain aa 318-349.
    Sequence:

    VPTARRPLSLLYYDRDSNIVKTDIPDMVVEAC


    Database link: P55103

  • Positive control
    • Human Liver tissue. Staining localises to hepatocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab73904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 38 kDa.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • Tissue specificity
    Expressed in benign prostatic hyperplasia.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activin beta C chain antibody
    • Activin beta-C chain antibody
    • IHBC antibody
    • Inhbc antibody
    • INHBC_HUMAN antibody
    • Inhibin beta C chain antibody
    • Inhibin, beta C antibody
    see all

Anti-Inhibin beta C chain antibody [betaC clone 1] images

  • ab73904 staining Inhibin beta C chain in human embryonal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000) for 16 hours at 4°C. An undiluted HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Inhibin beta C chain antibody [betaC clone 1] (ab73904)

This product has been referenced in:
  • Mellor SL  et al. Localization of activin beta(A)-, beta(B)-, and beta(C)-subunits in humanprostate and evidence for formation of new activin heterodimers of beta(C)-subunit. J Clin Endocrinol Metab 85:4851-8 (2000). Read more (PubMed: 11134153) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Odyssey Blocking Buffer as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample
Human Cell (COV434 cell line)
Specification
COV434 cell line
Permeabilization
Yes - Ethanol
Fixative
Formaldehyde
Username

Dr. Francesco Elia Marino

Verified customer

Submitted May 30 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
CAS Block Invitrogen as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (Human Embryonal Carcinoma)
Specification
Human Embryonal Carcinoma
Permeabilization
No
Fixative
Bouin's
Username

Dr. Francesco Elia Marino

Verified customer

Submitted May 02 2014

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Sample
Human Cell lysate - whole cell (COV434 cell pellet)
Specification
COV434 cell pellet
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. Francesco Elia Marino

Verified customer

Submitted Mar 12 2014

Thank you once again for your reply and enquriies.

I am very sorry to confirm that in this case we are regrettably not able to provide the custom formulations of these antibodies you have requested.

I can recommend you may like to...

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Thank you for your enquiry.

I am currently looking into whether this will be possible for you. I would appreciate if you could let me know how much of the ab7390 you require, what volume?

Many thanks for your cooperation. I look f...

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